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| Status |
Public on Jul 12, 2024 |
| Title |
Increased viral tolerance mediates by antiviral RNA interference in bat cells |
| Organisms |
Pteropus alecto; Tadarida brasiliensis; Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Bats harbor highly virulent viruses that can infect other mammals, including humans, posing questions about their immune tolerance mechanisms. Bat cells employ multiple strategies to limit virus replication and virus-induced immunopathology, but the coexistence of bats and fatal viruses remains poorly understood. Here, we investigated the antiviral RNA interference (RNAi) pathway in bat cells and discovered that they have an enhanced antiviral RNAi response, producing canonical viral small interfering RNAs (vsiRNAs) upon Sindbis virus (SINV) infection that were missing in human cells. Disruption of Dicer function resulted in increased viral load for three different RNA viruses in bat cells, indicating an interferon-independent antiviral pathway. Furthermore, our findings reveal the simultaneous engagement of Dicer and pattern-recognition receptors (PRRs), such as retinoic acid-inducible gene I (RIG-I), with double-stranded RNA, suggesting that Dicer attenuates the interferon response initiation in bat cells. These insights advance our comprehension of the distinctive strategies bats employ to coexist with viruses.
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| Overall design |
We performed small RNA-seq in virus-infected PaKi cells and human cells including HeLa and A549, we analyzed distribution of virus-derived small RNAs and mature microRNAs
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| Contributor(s) |
Dai Y, Wang B, wang J, Li Y |
| Citation missing |
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| |
| Submission date |
Dec 22, 2022 |
| Last update date |
Jul 13, 2024 |
| Contact name |
Yang Li |
| E-mail(s) |
yangli15@fudan.edu.cn
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| Organization name |
Fudan university
|
| Street address |
2005 Songhu Rd, Yangpu district
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| City |
Shanghai |
| ZIP/Postal code |
201601 |
| Country |
China |
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| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL15898 |
Illumina HiSeq 2000 (Pteropus alecto) |
| GPL34381 |
Illumina HiSeq 2000 (Tadarida brasiliensis) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA915074 |
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