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| Status |
Public on Dec 31, 2022 |
| Title |
RNA profile of nuclei isolated from neurons coexpressing VAChT and VGlut or VAChT and VGAT |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The concept of neurotransmitter identity, once considered singular and immutable for mature neurons, has been broadened by the recognition that many neurons release multiple neuro-active substances.
In order to demonstrate the extent of neurotransmitter co-expression, we labelled neuronal nuclei with UNC84-GFP using VAChT and either Vglut or VGAT split-GAL4s and isolated them with the isolation of nuclei tagged in specific ell types (INTACT) method
From the isolated nuclei, we isolated and sequenced Poly(A) RNA and examined the relative expression levels of glutamatergic, GABAergic, or cholinergic genes in each population.
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| Overall design |
Comparative gene expression profiling analysis of RNA-Seq data from INTACT purified nuclei from VAChT:Vglut split Gal4/UAS-UNC84-GFP and VAChT:VGAT split Gal4/UAS-UNC84-GFP flies
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| Contributor(s) |
Zhang Y, Chen N, River-Rodriguez EJ, Yu AD, Hobin M, Rosbash M, Griffith LC |
| Citation missing |
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| BioProject |
PRJNA914267 |
| |
| Submission date |
Dec 28, 2022 |
| Last update date |
Jan 03, 2023 |
| Contact name |
Albert Dayuan Yu |
| E-mail(s) |
adyu@brandeis.edu
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| Phone |
8579917778
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| Organization name |
Brandeis University
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| Department |
Molecular and Cell Biology
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| Lab |
Rosbash Lab
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| Street address |
415 South St.
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| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (4)
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