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| Status |
Public on May 31, 2023 |
| Title |
Comparative transcriptome analysis reveals metabolic differences between planktonic and biofilm of Actinobacillus pleuropneumoniae. |
| Organism |
Actinobacillus pleuropneumoniae serovar 1 str. 4074 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To reveal the transcriptional profiles of Actinobacillus pleuropneumoniae under biofilm and planktonic growth, we established a biofilm-forming culture method and constructed a mutant strain Δpga with defect in biofilm formation. Wild-type and Δpga mutant strains of Actinobacillus pleuropneumoniae strain 4074 were cultured in bottles with shaking for planktonic (WT_PK) and in microplates in static status for biofilm (WT_BF, Δpga), respectively. The bacteria in logarithmic growth period of different culture groups were collected for RNA seq.
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| Overall design |
The planktonic bacteria were obtained by culturing bacteria in bottles with shaking, and the biofilm were cultured in microplates in static status. Substances adhered to the bottom of the microplates were obtained as the biofilm layer or adhesion layer.
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| Contributor(s) |
Zhang Q, Li L |
| Citation(s) |
37237397 |
| |
| Submission date |
Dec 30, 2022 |
| Last update date |
May 31, 2023 |
| Contact name |
Qiuhong Zhang |
| E-mail(s) |
zhangqiuhong@webmail.hzau.edu.cn
|
| Phone |
13297978781
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| Organization name |
Huazhong Agricultural University
|
| Street address |
No. 1, Shizishan Street
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL32988 |
Illumina NovaSeq 6000 (Actinobacillus pleuropneumoniae serovar 1 str. 4074) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA916892 |
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