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| Status |
Public on Jul 01, 2024 |
| Title |
Transcriptional profiling of NK cells from tuberculosis patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
NK cells were purified from peripheral blood of healthy controls (n = 5), individuals with latent tuberculosis infection (n = 5), and active tuberculosis patients (n = 5). Total RNA was isolated from purified NK cells using RNA Isolation Kit (Dongsheng, R1061) according to the manufacturer's instructions. RNA Sequencing was conducted using the Illumina HiSeq X Ten platform (Novogene Bioinformatics Technology Co., Ltd.) with paired-end 150-bp reads.
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| Overall design |
Patients with ATB were diagnosed by significant symptoms of TB with a positive result of T-SPOT.TB test (a commercially available IGRA method) , and were further confirmed by Mtb culture of respiratory specimens. LTBI individuals were healthcare workers with positive T-SPOT.TB results but without any clinical TB symptoms. The individuals without known history of exposure to Mtb were included as healthy control (HC) subjects based on negative results of both T-SPOT.TB and PPD skin reactivity tests. ATB patients and LTBI individuals were recruited from Beijing Chest Hospital, a TB specialized hospital in China. All Hhealthy control individuals were recruited form Institute of Microbiology, Chinese Academy of Sciences. All participants aged ≥16 years, and had no significant other medical history such as human immunodeficiency virus (HIV) infection, cancers and diabetes.
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| Contributor(s) |
Liu CH |
| Citation(s) |
39030302 |
| BioProject |
PRJNA915785 |
| |
| Submission date |
Jan 02, 2023 |
| Last update date |
Oct 12, 2024 |
| Contact name |
lu z |
| E-mail(s) |
lu1457883143@gmail.com
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| Organization name |
institution of microbology
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| Lab |
liuch
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| Street address |
beijing
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| City |
beijing |
| State/province |
- 选择 - |
| ZIP/Postal code |
10010 |
| Country |
China |
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| Platforms (1) |
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| Samples (15)
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| GSM6911475 |
Active tuberculosis infection replicate 1 |
| GSM6911476 |
Active tuberculosis infection replicate 2 |
| GSM6911477 |
Active tuberculosis infection replicate 3 |
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