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Series GSE222228 Query DataSets for GSE222228
Status Public on Jan 18, 2023
Title The microRNomes of Chinese Hamster Ovary (CHO) cells and their extracellular vesicles, and how they respond to osmotic and ammonia stress
Organism Cricetulus griseus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary A new area of focus in Chinese Hamster Ovary (CHO) biotechnology is the role of small (exosomes) and large (microvesicles or microparticles) extracellular vesicles (EVs). CHO cells in culture exchange large quantities of proteins and RNA through these EVs, yet the content and role of these EVs remain elusive. MicroRNAs (miRs) are central to adaptive responses to stress and more broadly to changes in culture conditions. Given that EVs are highly enriched in miRs, and that EVs release large quantities of miRs both in vivo and in vitro, EVs and their miR content likely play an important role in adaptive responses. Here we report the miRNA landscape of CHO cells and their EVs under normal culture conditions and under ammonia and osmotic stress. We show that both cells and EVs are highly enriched in five miRs (among over 600 miRs) that make up about half of their total miR content, and that these highly enriched miRs differ significantly between normal and stress culture conditions. Notable is the high enrichment in mir-92a and miR-23a under normal culture conditions, in contrast to the high enrichment in let-7 family miRs (let-7c, let-7b and let-7a) under both stress conditions. The latter suggests a preserved stress-responsive function of the let-7 miR family, one of the most highly preserved miR families across species, where among other functions, let-7 miRs regulate core oncogenes, which, depending on the biological context, may tip the balance between cell cycle arrest and apoptosis. While the expected ­–based on their profound enrichment ­– important role of these highly enriched miRs remains to be dissected, our data and analysis constitute an important resource for exploring the role of miRs in cell adaptation as well as for synthetic applications.
 
Overall design Examination of small RNA profile of Chinese Hamster Ovary (CHO) cells, microparticles, and exosomes derived from standard and stressed (osmotic and ammonia) cultures via small RNA-Sequencing
 
Contributor(s) Jessica B, ET P
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Submission date Jan 05, 2023
Last update date Jan 18, 2023
Contact name Jessica Belliveau
E-mail(s) jbellive@udel.edu
Organization name University of Delaware
Department Dept. of Chemical and Biomolecular Engineering
Street address 590 Avenue 1743
City Newark
State/province DE
ZIP/Postal code 19713
Country USA
 
Platforms (1)
GPL32085 NextSeq 550 (Cricetulus griseus)
Samples (27)
GSM6918263 StandardCells_miRNA_biol rep 1 [Base_Cells_1_S7]
GSM6918264 StandardCells_miRNA_biol rep 2 [Base_Cells_2_S8]
GSM6918265 StandardCells_miRNA_biol rep 3 [Base_Cells_3_S9]
Relations
BioProject PRJNA918641

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Supplementary file Size Download File type/resource
GSE222228_counts-20221123_reformat.xlsx 1.0 Mb (ftp)(http) XLSX
GSE222228_expression-table-export-20221123.xlsx 2.7 Mb (ftp)(http) XLSX
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