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| Status |
Public on Aug 01, 2023 |
| Title |
Mycobacterial DNA binding protein 1 (MDP1) mediates gene regulation in Mycobacterium tuberculosis variant Bacillus Calmette-Guerin |
| Organism |
Mycobacterium tuberculosis variant bovis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the role of MDP1 in global transcription regulation, we established MDP1 conditional knockdown BCG using CRISPR/dCas9 system.
Transcriptome profiling was then performed using data obtained from RNA-seq of two BCG MDP1 mutants compared to the vector control at three growth time points.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for BCG Tokyo and its MDP1 cKD derivatives (MDP1_#1 cKD and MDP1_#2 cKD ).
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| Contributor(s) |
Shaban AK, Geberetsadik G, Mariko H, Inouchi E, Ozeki Y, Tateishi Y, Nishiyama A, Nishiuchi Y, Yamaguchi T, Takihara H, Okuda S, Matsumoto S |
| Citation(s) |
37644087 |
| |
| Submission date |
Jan 05, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Shaban Amina Kaboso |
| E-mail(s) |
kaboso90@gmail.com
|
| Organization name |
Niigata University
|
| Department |
Bacteriology
|
| Street address |
Asahimachi-Dori 1-757l, Chuo
|
| City |
Niigata |
| ZIP/Postal code |
951-8510 |
| Country |
Japan |
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| Platforms (1) |
| GPL33005 |
Illumina NovaSeq 6000 (Mycobacterium tuberculosis variant bovis) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA918672 |
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