NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE222264 Query DataSets for GSE222264
Status Public on Mar 04, 2024
Title Phase separation-competent FBL shapes nucleolar function and translation in leukemogenesis (RNA-Seq)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Exploring the mechanism of transformation and propagation of acute myeloid leukemia is still the key to solving its refractory and relapse-prone problems. Phase separation refers to the process of specific aggregation of biological macromolecules through multivalent binding, and then leads to the functional sectorization in cell. Despite the fact that phase separation has been related to the dysregulation of various cellular processes, its role in the malignant transformation and development of AML is still less characterized. Nucleolus, as a multilayer condensate formed by phase separation, is an important place for the initiation of ribosome formation, and its function is directly related to protein translation. High-resolution imaging and fluorescence recovery after photo-bleaching experiments decode the positive regulatory relationship between the phase separation of FBL and the structure and function of nucleoli. Here, we used polysome profiling coupled with RNA-seq to depict transcriptome of AML cells with or without FBL knockdown.
 
Overall design Polysome profiling coupled with RNA-seq to depict transcriptome of AML cells with or without FBL knockdown.
 
Contributor(s) Yang L, Jiang P, Qian P
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 05, 2023
Last update date Mar 05, 2024
Contact name Jiang Penglei
E-mail(s) jpl3982233@zju.edu.cn
Organization name Zhejiang University
Department School of basic medicine science
Street address Yuhangtang Road 866
City Hangzhou
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM6919265 polysome RNA CT1
GSM6919266 polysome RNA CT2
GSM6919267 polysome RNA U1
This SubSeries is part of SuperSeries:
GSE222266 Phase separation-competent FBL shapes nucleolar function and translation in leukemogenesis
Relations
BioProject PRJNA918713

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE222264_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.