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| Status |
Public on Jul 05, 2023 |
| Title |
Generation and functional analysis of defective viral genomes during SARS-CoV-2 infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we elucidated defective viral genome generation in SARS-CoV-2 and its relationship with host antiviral immune response. We observed DVGs ubiquitously from RNA-seq datasets of in vitro infections and autopsy lung tissues of COVID-19 patients.
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| Overall design |
Gene expression profiling analysis of RNA-seq data of primary lung epithelial cells from donors of different age groups cultured on air-liquid interface for 4-5 weeks before infected with SARS-CoV-2. Samples were collected at 2-3 timepoints post infection.
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| Contributor(s) |
Osborn RM, Anderson CS, Gilliam NJ, Zhou T, Li S, Spaudau S, Huang L, Matthews D, Dewhurst S, Thakar J, Sun Y, Mariani TJ |
| Citation(s) |
37074178 |
| |
| Submission date |
Jan 08, 2023 |
| Last update date |
Jul 05, 2023 |
| Contact name |
Raven M. Osborn |
| E-mail(s) |
Raven_Osborn@URMC.rochester.edu
|
| Phone |
8166683462
|
| Organization name |
University of Rochester School of Medicine and Dentistry
|
| Department |
Microbiology
|
| Lab |
Juilee Thakar and Stephen Dewhurst
|
| Street address |
601 Elmwood Ave
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA921844 |
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