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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 10, 2023 |
| Title |
Screening and identification of miR-181a-5p in oral squamous cell carcinoma and functional verification in vivo and in vitro |
| Organism |
Cricetulus griseus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Background: Oral squamous cell carcinoma (OSCC) is a common malignant tumor associated with poor prognosis. MicroRNAs (miRNAs) play crucial regulatory roles in the cancer development. However, the role of miRNAs in OSCC development and progression is not well understood. Methods: We sought to establish a dynamic Chinese hamster OSCC animal model, construct miRNA differential expression profiles of its occurrence and development, predict its targets, and perform functional analysis and validation in vitro. Results: Using expression and functional analyses, the key candidate miRNA (miR-181a-5p) was selected for further functional research, and the expression of miR-181a-5p in OSCC tissues and cell lines was detected. Subsequently, transfection technology and a nude mouse tumorigenic model were used to explore potential molecular mechanisms. miR-181a-5p was significantly downregulated in human OSCC specimens and cell lines, and decreased miR-181a-5p expression was observed in multiple stages of the Chinese hamster OSCC animal model. Moreover, upregulated miR-181a-5p significantly inhibited OSCC cell proliferation, colony formation, invasion, and migration; blocked the cell cycle; and promoted apoptosis. BCL2 was identified as a target of miR-181a-5p. BCL2 may interact with apoptosis- (BAX), invasion- and migration- (TIMP1, MMP2, and MMP9), and cell cycle-related genes (KI67, E2F1, CYCLIND1, and CDK6) to further regulate biological behavior. Tumor xenograft analysis indicated that tumor growth was significantly inhibited in the high miR-181a-5p expression group. Conclusions: Our findings indicate that miR-181a-5p can be used as a potential biomarker and provide a novel animal model for mechanistic research on oral cancer.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for the Chinese hamster oral cancer animal model (Use DMBA to induce oral cancer in the animal model)
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| Contributor(s) |
Xu G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 09, 2023 |
| Last update date |
Jan 11, 2023 |
| Contact name |
Guohua Song |
| E-mail(s) |
ykdsgh@sxmu.edu.cn
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| Organization name |
Shanxi Medical University
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| Department |
Laboratory Animal Center
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| Lab |
Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model
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| Street address |
Road Xinjian 56, Taiyuan 030001, China
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| City |
Taiyuan |
| State/province |
Shanxi |
| ZIP/Postal code |
030001 |
| Country |
China |
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| Platforms (1) |
| GPL20904 |
Illumina HiSeq 2500 (Cricetulus griseus) |
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| Samples (12)
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| GSM6923186 |
buccal pouch, no treatment (control), rep 1 |
| GSM6923187 |
buccal pouch, no treatment (control), rep 2 |
| GSM6923188 |
buccal pouch, no treatment (control), rep 3 |
| GSM6923189 |
buccal pouch,coated with DMBA (simple hyperplasia), rep 1 |
| GSM6923190 |
buccal pouch,coated with DMBA (simple hyperplasia), rep 2 |
| GSM6923191 |
buccal pouch,coated with DMBA (simple hyperplasia), rep 3 |
| GSM6923192 |
buccal pouch,coated with DMBA (abnormal hyperplasia), rep 1 |
| GSM6923193 |
buccal pouch,coated with DMBA (abnormal hyperplasia), rep 2 |
| GSM6923194 |
buccal pouch,coated with DMBA (abnormal hyperplasia), rep 3 |
| GSM6923195 |
buccal pouch,coated with DMBA (squamous cell carcinoma), rep 1 |
| GSM6923196 |
buccal pouch,coated with DMBA (squamous cell carcinoma), rep 2 |
| GSM6923197 |
buccal pouch,coated with DMBA (squamous cell carcinoma), rep 3 |
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| Relations |
| BioProject |
PRJNA922080 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222429_miRNA_exp_count_matrix.csv.gz |
4.7 Kb |
(ftp)(http) |
CSV |
| GSE222429_miRNA_exp_tpm_matrix.csv.gz |
6.6 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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