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| Status |
Public on Jun 23, 2024 |
| Title |
Immune landscape of Isocitrate Dehydrogenase (IDH) stratified human gliomas [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The brain tumor immune microenvironment (TIME) continuously evolves during glioma progression, but only a limited view of a highly complex glioma associated immune contexture across isocitrate dehydrogenase mutation (IDH) classified gliomas is known. Herein, we present an unprecedentedly comprehensive view of myeloid and lymphoid cell type diversity with our m-RNA sequencing interrogation.
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| Overall design |
The glioma associated CD45+ (n=15) and CD45hi (n=3) and CD45lo (n=3) leukocyte fractions were subjected to Fluorescence-activated cell sorting (FACS) from cryopreserved single cells. The leukocyte enriched single cell suspensions were obtained from freshly resected tumors of IDH-mutant primary (IMP; n=3), IDH-mutant recurrent (IMR; n=4), IDH-wild type primary (IWP; n=4), or IDH-wild type recurrent (IWR; n=4) glioma patients and quasi-normal non-glioma brain (NGB; n=1) from a dysembryoplastic neuroepithelial tumor.
*** Raw data are not provided for this study. Raw data are protected by MD Anderson internal review board (IRB)-approved protocol numbers LAB03-0687, LAB04-0001 and 2012-0441 and Baylor College of Medicine IRB-approved protocol number H-13798. ***
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| Contributor(s) |
Gupta P, Dang M, Wang L, Bhat K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 10, 2023 |
| Last update date |
Jun 24, 2024 |
| Contact name |
Jeffrey Andrew How |
| E-mail(s) |
jahow@mdanderson.org
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| Organization name |
The University of Texas MD Anderson Cancer Center
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| Department |
Gyn Onc & Reproductive Med
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| Street address |
1515 Holcombe Blvd. Unit 1362
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030-4009 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (21)
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| GSM6925351 |
IMP4, replicate1, mRNASeq |
| GSM6925352 |
IMR1, replicate1, mRNASeq |
| GSM6925353 |
IMR2, replicate1, mRNASeq |
| GSM6925354 |
IMR3, replicate1, mRNASeq |
| GSM6925355 |
IMR4, replicate1, mRNASeq |
| GSM6925356 |
IWP1, replicate1, mRNASeq |
| GSM6925357 |
IWP2, replicate1, mRNASeq |
| GSM6925358 |
IWP3, replicate1, mRNASeq |
| GSM6925359 |
IWR1, replicate1, mRNASeq |
| GSM6925360 |
IWR2, replicate1, mRNASeq |
| GSM6925361 |
IWR3, replicate1, mRNASeq |
| GSM6925362 |
IWR4, replicate1, mRNASeq |
| GSM6925363 |
IMP3_CD45 hi, replicate1 |
| GSM6925364 |
IMR2_CD45 hi, replicate1 |
| GSM6925365 |
IWR5_CD45 hi, replicate1 |
| GSM6925366 |
IMP3_CD45 lo, replicate1 |
| GSM6925367 |
IMR2_CD45 lo, replicate1 |
| GSM6925368 |
IWR5_CD45 lo, replicate1 |
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| This SubSeries is part of SuperSeries: |
| GSE222522 |
Immune landscape of Isocitrate Dehydrogenase (IDH) stratified human gliomas |
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| Relations |
| BioProject |
PRJNA922462 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222518_RAW.tar |
4.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data provided as supplementary file |
| Raw data not provided for this record |
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