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Series GSE222930 Query DataSets for GSE222930
Status Public on Aug 01, 2023
Title A somatic cell cloned rhesus monkey obtained by trophoblast replacement [WGBS I]
Organisms Macaca fascicularis; Macaca mulatta
Experiment type Methylation profiling by high throughput sequencing
Summary Somatic cell nuclear transfer (SCNT) has been used to clone cynomolgus monkeys (Macaca fascicularis), but the cloning of other non-human primate species remains to be achieved. On the other hand, our histological examination indicated severe calcification of the placenta of SCNT fetuses. Additionally, we found that some of the maternal-biased imprinting genes were aberrantly lost in the cloned embryos by multi-omics analysis. We thus developed a trophoblast replacement (TR) method, providing an ICSI-derived placenta for the SCNT fetus, to support the full-term development of the cloned embryos after implantation. By combining this TR method with treatment with epigenetic modification factors, we obtained a healthy somatic cell cloned rhesus monkey. Thus, TR represents a useful approach for rhesus monkey cloning.
Overall design Whole genome bisulfite sequencing
Contributor(s) Liao Z
Citation(s) 38228612
Submission date Jan 15, 2023
Last update date Jan 29, 2024
Contact name Liao Zhaodi
Phone 17521632708
Organization name Chinese Academy of Sciences
Department Institute of Neuroscience
Street address 500, Qiangye Road
City Shanghai
ZIP/Postal code 2016000
Country China
Platforms (2)
GPL32224 DNBSEQ-G400 (Macaca mulatta)
GPL32974 DNBSEQ-G400 (Macaca fascicularis)
Samples (20)
GSM6935486 WGBS Placenta_WGBS_ICSI_rep1
GSM6935487 WGBS Placenta_WGBS_ICSI_rep2
GSM6935488 WGBS Placenta_WGBS_ICSI_rep3
This SubSeries is part of SuperSeries:
GSE239746 A somatic cell cloned rhesus monkey obtained by trophoblast replacement
BioProject PRJNA924042

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE222930_RAW.tar 7.8 Gb (http)(custom) TAR (of BW, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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