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| Status |
Public on Mar 31, 2023 |
| Title |
Expression data from Trigonelline-treated SAMP8 mice Hippocampus |
| Platform organism |
Mus musculus |
| Sample organism |
Mus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals neuroprotective and antiinflammatory effects of TG in SAMP8 (aging model ) mice Hippocampus thus helps in acelerating learning and memory restoration process.
by suppressing proinflammatory cytokine related genes and thus accekerate neurotransmitter release in SAMP8 aging model mice hippocampus
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Clariom S assay system (Thermo Fisher, Japan) was used on duplicate RNA samples of each group. In accordance with the user manual, complementary DNA (cDNA) for microarray was generated using the GeneChip WT Plus Reagent kit (Thermo Fisher Science, Japan). Samples were hybridized using Clariom S GeneChip microarray kit (Thermo Fisher Science, Japan). After washing and staining, images were captured, and then CEL data were generated using the GeneChip Scanner 3000 (Thermo Fisher Science, Japan). The raw CEL data were normalized using the transcriptome analysis console (TAC) software ver.4.0.1 following the SST-robust multichip average (SST-RMA) algorithm (Thermo Fisher Scientific, Japan).
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| Contributor(s) |
Sharmin A |
| Citation(s) |
37721682 |
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| Submission date |
Jan 20, 2023 |
| Last update date |
Feb 14, 2024 |
| Contact name |
Farhana Ferdousi |
| E-mail(s) |
farhana_ferdousi24@yahoo.com
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| Organization name |
University of Tsukuba
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| Street address |
Tennodai 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8575 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA925923 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223398_RAW.tar |
10.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE223398_SAMP8_TGvsSAMP8.txt.gz |
700.4 Kb |
(ftp)(http) |
TXT |
| GSE223398_SAMP8vsSAMR1.txt.gz |
702.7 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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