 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 31, 2023 |
| Title |
Characterization of radiation‑resistance mechanism in Spirosoma montaniterrae DY10T in terms of transcriptional regulatory system |
| Organism |
Spirosoma montaniterrae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Spirosoma montaniterrae DY10T is an uncharacterized radiation-resistanct bacteria isolated from soil in South Korea. In order to identify the underlying mechanisms involved in UVC-resistance, we generated time-series transcriptome data with UVC radiation and analyzed data with computational methods.
|
| |
|
| Overall design |
The bacterial strain DY10T (KCTC 23999T) was grown at 25ºC in liquid nutrient-rich medium tryptone glucose yeast extract (TGY; 1% tryptone, 0.1% glucose and 0.5% yeast extract) or TGY agar plates. For the UVC treatment, the early stationary phase cells on TGY agar plates were exposed to 600 JM-2 of ultraviolet using an ultraviolet crosslinker (UVP, CX-2000, CA, USA) at 254nm. The post-radiation cells of UVC were collected by centrifugation (17,500 × g for 5 min) at 5min, 1hr, 3hr and 12hr with washing and stored with RNAlater solution at -80ºC. Total RNA was isolated using the Ambion Ribopure-Yeast RNA kit, according to manufacturer's instructions.
|
| Web link |
https://www.nature.com/articles/s41598-023-31509-8
|
| |
|
| Contributor(s) |
Cho C, Lee D, Jeong D, Kim S, Kim MK, Srinivasan S |
| Citation(s) |
36959250 |
| |
| Submission date |
Jan 24, 2023 |
| Last update date |
Apr 03, 2023 |
| Contact name |
Changyun Cho |
| E-mail(s) |
joebrother@snu.ac.kr
|
| Organization name |
Seoul National University
|
| Street address |
1, Gwanak-ro, Gwanak-gu
|
| City |
seoul |
| State/province |
Not US or Canada |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL33055 |
Illumina NextSeq 500 (Spirosoma montaniterrae) |
|
| Samples (5)
|
| GSM6965270 |
Spirosoma montanitarrae DY10T, UVC, control |
| GSM6965271 |
Spirosoma montanitarrae DY10T, UVC, 5 min |
| GSM6965272 |
Spirosoma montanitarrae DY10T, UVC, 1 hr |
| GSM6965273 |
Spirosoma montanitarrae DY10T, UVC, 3 hr |
| GSM6965274 |
Spirosoma montanitarrae DY10T, UVC, 12 hr |
|
| Relations |
| BioProject |
PRJNA926964 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223604_Spirosoma_UVC_read_counts.csv.gz |
61.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...