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| Status |
Public on Mar 01, 2024 |
| Title |
Effect of hypoxia on canine glioma stem-like cells metabolism |
| Organism |
Canis lupus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
To investigate the role of hypoxia treatment on the metabolic profiles of canine glioma cells
We then performed reduce representation bisulfite sequencing on 3 unique canine cell lines and correlated with functional assays
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| Overall design |
Following hypoxia treatment, migration, viability, gene expression, and RRBS profiling was carried out to understand the role of metabolism-related changes in canine glioma cells
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| Contributor(s) |
Toedebusch C, Toedebusch R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 27, 2023 |
| Last update date |
Mar 02, 2024 |
| Contact name |
Ryan Toedebusch |
| E-mail(s) |
ryantoedebusch@gmail.com
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| Organization name |
University of California
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| Street address |
1 Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL33062 |
Illumina HiSeq 2500 (Canis lupus) |
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| Samples (18)
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| GSM6997975 |
Sample 4: 0514 GS cells, normoxia, RRBS |
| GSM6997976 |
Sample 5: 0514 GS cells, normoxia, RRBS |
| GSM6997977 |
Sample 6: 0514 GS cells, normoxia, RRBS |
| GSM6997978 |
Sample 7: 1110 GS cells, normoxia, RRBS |
| GSM6997979 |
Sample 8: 1110 GS cells, normoxia, RRBS |
| GSM6997980 |
Sample 9: 1110 GS cells, normoxia, RRBS |
| GSM6997981 |
Sample 10: G06 cells, hypoxia, RRBS |
| GSM6997982 |
Sample 11: G06 cells, hypoxia, RRBS |
| GSM6997983 |
Sample 12: G06 cells, hypoxia, RRBS |
| GSM6997984 |
Sample 13: 0514 GS cells, hypoxia, RRBS |
| GSM6997985 |
Sample 14: 0514 GS cells, hypoxia, RRBS |
| GSM6997986 |
Sample 15: 0514 GS cells, hypoxia, RRBS |
| GSM6997987 |
Sample 16: 1110 GS cells, hypoxia, RRBS |
| GSM6997988 |
Sample 17: 1110 GS cells, hypoxia, RRBS |
| GSM6997989 |
Sample 18: 1110 GS cells, hypoxia, RRBS |
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| Relations |
| BioProject |
PRJNA928670 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223852_RAW.tar |
933.6 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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