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Series GSE224210 Query DataSets for GSE224210
Status Public on Mar 21, 2023
Title Single cell and spatial transcriptomic profile of whole CLAD and donor control lung tissue
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Chronic lung allograft dysfunction (CLAD) is the leading cause of death in lung transplant recipients. CLAD is characterized clinically by a persistent decline in pulmonary function and histologically by the development of airway centered fibrosis known as bronchiolitis obliterans. We performed single-cell sequencing and spatial transcriptomic analysis of explanted tissues from human lung recipients with CLAD and donor control lungs.
 
Overall design For the single cell dataset, whole lung tissue segments from four CLAD samples and three donor control samples were used. For the spatial transcriptomic dataset, tissue blocks from three CLAD samples and one donor control sample were used. Donor control tissue was obtained from donor lungs used for transplant that were harvested, perserved, implanted, and then trimmed to fit the recipient chest cavity with excess donor trimming preserved for research. Donor control lung tissue underwent formal pahtology evaluation that confirmed the absence of any significant lung disease.
 
Contributor(s) Scott P, Aaditya K, Vaibhav J, Jamie T
Citation(s) 36946463
NIH grant(s)
Grant ID Grant title Affiliation Name
R38 HL143612 Duke Resident Physician-Scientist Program- NHLBI DUKE UNIVERSITY Scott M Palmer
U01 AI163099 Lung Transplant Clinical Trial Network (LT-CTN) DUKE UNIVERSITY Scott M Palmer
R21 TR003037 Proof of concept treatment interventions in a rodent model of the rare disease bronchiolitis obliterans DUKE UNIVERSITY Scott M Palmer
Submission date Feb 01, 2023
Last update date Mar 23, 2023
Contact name Scott Palmer
E-mail(s) scott.palmer@duke.edu
Organization name Duke University
Street address 2100 Erwin Road
City Durham
State/province NC
ZIP/Postal code 27705
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (11)
GSM7017450 CLAD sample 1
GSM7017451 CLAD sample 2
GSM7017452 CLAD sample 3
Relations
BioProject PRJNA930324

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224210_Cell_Identities.csv.gz 534.5 Kb (ftp)(http) CSV
GSE224210_Normalized_Count_Matrix.csv.gz 113.5 Mb (ftp)(http) CSV
GSE224210_RAW.tar 480.2 Mb (http)(custom) TAR (of CLOUPE)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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