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Series GSE224326 Query DataSets for GSE224326
Status Public on Mar 08, 2023
Title Distinct differences in immunological properties of equine orthobiologics revealed by functional and transcriptomic analysis using an activated macrophage readout system
Organism Equus caballus
Experiment type Expression profiling by high throughput sequencing
Summary Multiple biological therapies for orthopedic injuries are marketed to veterinarians, despite a lack of rigorous comparative biological activity data to guide informed decisions in selecting a most effective compound. Therefore, the goal of this study was to use relevant bioassay systems to directly compare the anti-inflammatory and immunomodulatory activity of three commonly used orthobiological therapies (OTs): mesenchymal stromal cells (MSC), autologous conditioned serum (ACS), and platelet rich plasma (PRP). Equine monocyte-derived macrophages were used as the readout system to compare therapies, including cytokine production and transcriptomic responses. 
 
Overall design Macrophages were stimulated with IL-1ß and treated 24 hours with orthobiologicss, washed and cultured an additional 24 hours to generate supernatants. Secreted cytokines were measured by multiplex immunoassay and ELISA. To assess global transcriptomic responses to treatments, RNA was extracted from macrophages and subjected to full RNA sequencing
 
Contributor(s) Chow L, Pezzanite L, Dow S, Goodrich L
Citation(s) 36876001
Submission date Feb 02, 2023
Last update date Mar 08, 2023
Contact name Steven Dow
E-mail(s) sdow@colostate.edu
Organization name Colorado State University
Department Clinical Sciences
Lab ACC 254
Street address 300 W Drake Rd
City Fort Collins
State/province CO
ZIP/Postal code 80524
Country USA
 
Platforms (1)
GPL26749 Illumina NovaSeq 6000 (Equus caballus)
Samples (15)
GSM7019472 Mc1-PRP-1
GSM7019473 Mc1-PRP-2
GSM7019474 Mc1-PRP-3
Relations
BioProject PRJNA930645

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224326_Partek_Equine_M0.txt.gz 1.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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