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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 21, 2023 |
| Title |
Gene expression changes in mice induced by parabiosis and recovery [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Aging is classically conceptualized as an ever-increasing trajectory of damage accumulation and loss of function, leading to increases in morbidity and mortality. However, recent in vitro studies have raised the possibility of age reversal. We characterized several models in which biological age is perturbed. Heterochronic parabiosis and recovery from this procedure is one such example.
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| Overall design |
We studied a mouse model of heterochronic parabiosis. Mouse pairs (either age-mismatched, heterochronic; or age-matched, isochronic) were surgically joined and maintained for 1-3 months. After this period, a subset of mice were euthanized and tissues were collected for analysis, and another subset of pairs was surgically detached and allowed to recover for 2 months, after which tissues were collected. We sequenced RNA isolated from these samples to analyze gene expression changes.
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| Contributor(s) |
Poganik JR, Zhang B, Baht GS, Tyshkovskiy A, Deik A, Kerepesi C, Yim SH, Lu AT, Haghani A, Gong T, Hedman AM, Andolf E, Lee DE, Trapp A, Bareja A, McKay LK, Shindyapina AV, Dmitriev SE, Pershagen G, Almqvist C, Clish CB, Horvath S, White JP, Gladyshev VN |
| Citation(s) |
37086720, 37500973 |
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| Submission date |
Feb 02, 2023 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Jesse Poganik |
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
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| Department |
Medicine/Genetics
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (39)
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| This SubSeries is part of SuperSeries: |
| GSE224447 |
DNA methylation and gene expression changes in mice induced by parabiosis and recovery |
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| Relations |
| BioProject |
PRJNA930740 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE224378_RAW.tar |
127.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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