 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 20, 2023 |
| Title |
SARS-CoV-2 airway infection results in time-dependent sensory abnormalities in a hamster model |
| Organism |
Mesocricetus auratus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Despite being largely confined to the airways, SARS-CoV-2 infection has been associated with sensory abnormalities that manifest in both acute and long-lasting phenotypes. To gain insight on the molecular basis of these sensory abnormalities, we used the golden hamster infection model to characterize the effects of SARS-CoV-2 versus Influenza A virus (IAV) infection on the sensory nervous system. SARS-CoV-2-infected hamsters demonstrated mechanical hypersensitivity during acute infection; intriguingly, this hypersensitivity was milder, but prolonged when compared to IAV-infected hamsters. RNA sequencing (RNA-seq) of thoracic DRGs from acute infection revealed predominantly neuron-biased signaling perturbations in SARS-CoV-2-infected animals as opposed to type I interferon signaling in tissue derived from IAV-infected animals. RNA-seq of 31dpi thoracic DRGs from SARS-CoV-2-infected animals highlighted a uniquely neuropathic transcriptomic landscape, which was consistent with substantial SARS-CoV-2-specific mechanical hypersensitivity at 28dpi. Overall, this work elucidates novel transcriptomic signatures triggered by SARS-CoV-2 that may underlie both short- and long-term sensory abnormalities while also highlighting several therapeutic targets for alleviation of infection-induced hypersensitivity.
|
| |
|
| Overall design |
RNA-sequencing of SARS-CoV-2-, IAV-, and mock-treated hamster dorsal root ganglion tissues at 1 and 4 days-post-infection to allow differential expression analysis among infection groups and time points. RNA-sequencing of SARS-CoV-2 and mock-treated hamster dorsal root ganglion tissues at 31 days-post-infection to allow differential expression analysis.
|
| |
|
| Contributor(s) |
Frere JJ, Serafini RA, Zachariou V, tenOever BR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 07, 2023 |
| Last update date |
Mar 22, 2023 |
| Contact name |
Justin J Frere |
| E-mail(s) |
justinjfrere@gmail.com
|
| Phone |
4802412072
|
| Organization name |
NYU Langone Health
|
| Department |
Microbiology
|
| Lab |
tenOever Lab
|
| Street address |
Alexandria Center for Life Sciences, West Tower, 430 E 29th St.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL24490 |
Illumina NextSeq 500 (Mesocricetus auratus) |
| GPL28997 |
Illumina NovaSeq 6000 (Mesocricetus auratus) |
|
| Samples (30)
|
| GSM7030092 |
Dorsal Root Ganglion, MOCK, 1dpi, 4 |
| GSM7030093 |
Dorsal Root Ganglion, SARS-CoV-2, 1dpi, 1 |
| GSM7030094 |
Dorsal Root Ganglion, SARS-CoV-2, 1dpi, 2 |
| GSM7030095 |
Dorsal Root Ganglion, SARS-CoV-2, 1dpi, 3 |
| GSM7030096 |
Dorsal Root Ganglion, SARS-CoV-2, 1dpi, 4 |
| GSM7030097 |
Dorsal Root Ganglion, IAV, 1dpi, 1 |
| GSM7030098 |
Dorsal Root Ganglion, IAV, 1dpi, 2 |
| GSM7030099 |
Dorsal Root Ganglion, IAV, 1dpi, 3 |
| GSM7030100 |
Dorsal Root Ganglion, IAV, 1dpi, 4 |
| GSM7030101 |
Dorsal Root Ganglion, MOCK, 4dpi, 1 |
| GSM7030102 |
Dorsal Root Ganglion, MOCK, 4dpi, 2 |
| GSM7030103 |
Dorsal Root Ganglion, MOCK, 4dpi, 3 |
| GSM7030104 |
Dorsal Root Ganglion, MOCK, 4dpi, 4 |
| GSM7030105 |
Dorsal Root Ganglion, SARS-CoV-2, 4dpi, 1 |
| GSM7030106 |
Dorsal Root Ganglion, SARS-CoV-2, 4dpi, 2 |
| GSM7030107 |
Dorsal Root Ganglion, SARS-CoV-2, 4dpi, 3 |
| GSM7030108 |
Dorsal Root Ganglion, SARS-CoV-2, 4dpi, 4 |
| GSM7030109 |
Dorsal Root Ganglion, IAV, 4dpi, 1 |
| GSM7030110 |
Dorsal Root Ganglion, IAV, 4dpi, 2 |
| GSM7030111 |
Dorsal Root Ganglion, IAV, 4dpi, 3 |
| GSM7030112 |
Dorsal Root Ganglion, IAV, 4dpi, 4 |
| GSM7030113 |
Dorsal Root Ganglion, SARS-CoV-2, 31dpi, 1 |
| GSM7030114 |
Dorsal Root Ganglion, SARS-CoV-2, 31dpi, 2 |
| GSM7030115 |
Dorsal Root Ganglion, SARS-CoV-2, 31dpi, 3 |
| GSM7030116 |
Dorsal Root Ganglion, MOCK, 31dpi, 1 |
| GSM7030117 |
Dorsal Root Ganglion, MOCK, 31dpi, 2 |
| GSM7030118 |
Dorsal Root Ganglion, MOCK, 31dpi, 3 |
|
| Relations |
| BioProject |
PRJNA932256 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE224710_RAW.tar |
8.4 Mb |
(http)(custom) |
TAR (of SF) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...