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| Status |
Public on May 01, 2023 |
| Title |
RNA-seq data analysis of TgAP2XII-1 on Toxoplasma gondii merozoites development |
| Organism |
Toxoplasma gondii |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate transcriptional role of TgAP2XII-1 on type I Toxoplasma gondii (RHΔhxgprt Tir1), an auxin inducible degron system was introduced to control the protein expression of TgAP2XII-1 and the target protein could be totally degradated in 3h after adding IAA.
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| Overall design |
Transgenic parasite iKD AP2XII-1-mAID-3HA was treated with IAA for 0h, 12h or 24h, then total RNA were extracted for deep sequencing.
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| Contributor(s) |
Fan F, Shen B |
| Citation(s) |
37750704 |
| |
| Submission date |
Feb 09, 2023 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Bang Shen |
| E-mail(s) |
shenbang@mail.hzau.edu.cn
|
| Phone |
+86-27-87281810
|
| Organization name |
Huazhong Agricultural University
|
| Street address |
No. 1 SHizishan St
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL33110 |
MGISEQ-2000RS (Toxoplasma gondii) |
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| Samples (9)
|
| GSM7035880 |
iKD AP2XII-1 +IAA 0h, biological repeat 1 |
| GSM7035881 |
iKD AP2XII-1 +IAA 0h, biological repeat 2 |
| GSM7035882 |
iKD AP2XII-1 +IAA 0h, biological repeat 3 |
| GSM7035883 |
iKD AP2XII-1 +IAA 12h, biological repeat 1 |
| GSM7035884 |
iKD AP2XII-1 +IAA 12h, biological repeat 2 |
| GSM7035885 |
iKD AP2XII-1 +IAA 12h, biological repeat 3 |
| GSM7035886 |
iKD AP2XII-1 +IAA 24h, biological repeat 1 |
| GSM7035887 |
iKD AP2XII-1 +IAA 24h, biological repeat 2 |
| GSM7035888 |
iKD AP2XII-1 +IAA 24h, biological repeat 3 |
|
| Relations |
| BioProject |
PRJNA933144 |
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