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| Status |
Public on Jul 01, 2010 |
| Title |
RpoE regulon in Rhodobacter sphaeroides |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
In Rhodobacter sphaeroides a transcriptional response to the reactive oxygen species singlet oxygen is controlled by the group IV sigma factor RpoE and the anti-sigma factor ChrR. In this study, we integrated various large datasets to identify genes within the singlet oxygen stress response that contain RpoE-dependent promoters within R. sphaeroides. Transcript pattern clustering and a RpoE-binding sequence model were used to predict candidate promoters that respond to singlet oxygen stress in R. sphaeroides. These candidate promoters were experimentally validated to nine R. sphaeroides RpoE-dependent promoters that control the transcription of 15 genes activated by singlet oxygen.
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| Overall design |
DNA immunoprecipitated with polyclonal antibodies against RpoE or the Beta' subunit of RNA polymerase was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.
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| Contributor(s) |
Dufour YS, Donohue TJ, Landick R |
| Citation(s) |
18723027 |
| |
| Submission date |
Jun 25, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Yann S Dufour |
| Organization name |
University of Wisconsin - Madison
|
| Department |
Bacteriology
|
| Lab |
Timothy Donohue
|
| Street address |
1550 Linden Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10463 |
UW_Rhodobacter_sphaeroides_2.4.1_385K_v2 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA128415 |
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