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Series GSE22585 Query DataSets for GSE22585
Status Public on Jun 29, 2011
Title Genome-wide profiling of diel and circadian gene expression of the malaria vector Anopheles gambiae
Platform organisms Plasmodium falciparum; Anopheles gambiae
Sample organism Anopheles gambiae
Experiment type Expression profiling by array
Summary Anopheles gambiae, the primary African malarial mosquito, exhibits numerous behaviors that are under diel and circadian control, including locomotor activity, swarming, mating, host seeking, eclosion, egg laying and sugar feeding. However, little has been performed to elucidate the molecular basis for these daily rhythms. To study how gene expression is globally regulated by diel and circadian mechanisms, we have undertaken a DNA microarray analysis of A. gambiae head and bodies under 12:12 light:dark cycle (LD) and constant dark (DD, free-running) conditions. Zeitgeber Time (ZT) with ZT12 defined as time of lights OFF under the light:dark cycle, and ZT0 defined as end of the dawn transition. Circadian Time (CT) with CT0 defined as subjective dawn, inferred from ZT0 of the previous light:dark cycle.
 
Overall design Adult mated but non-blood fed female mosquito heads and bodies under 12:12 light:dark cycle (LD) and constant dark (DD) conditions were collected every 4 hr to generate 48 hr gene expression profiles, and samples were processed with Affymetrix full genome microarrays. Downstream analysis identified genes with ~24hr rhythmic expression profiles.
Web link http://nd.edu/~bioclock/
 
Contributor(s) Rund SS, Hou TY, Ward SM, Collins FH, Duffield GE
Citation(s) 21715657
Submission date Jun 25, 2010
Last update date Jan 24, 2013
Contact name Samuel S.C. Rund
E-mail(s) srund@nd.edu
Phone 1-574-631-1869
URL http://www.nd.edu/~srund
Organization name University of Notre Dame
Department Biological Science
Lab Duffield
Street address Galvin Life Science
City Notre Dame
State/province IN
ZIP/Postal code 46556
Country USA
 
Platforms (1)
GPL1321 [Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array
Samples (104)
GSM560414 heads-dark_dark-CircadianTime0-rep1
GSM560415 heads-dark_dark-CircadianTime0-rep2
GSM560416 heads-dark_dark-CircadianTime4-rep1
Relations
BioProject PRJNA128393

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22585_RAW.tar 201.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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