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| Status |
Public on Apr 04, 2024 |
| Title |
Maternal gestational nutrition perturbs offspring sperm epigenome in sheep [BiSulfite-seq] |
| Organism |
Ovis aries |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
There is a growing body of evidence that inadequate maternal nutrition during gestation can have immediate and life-long effects on offspring. However, little is known about the reproductive effects of maternal gestational nutrition in offspring males. Here, using a sheep model of poor maternal nutrition (restricted- or over-feeding) during gestation, we found that poor maternal gestational nutrition does not affect semen characteristics (i.e. volume, sperm concentration, pH, sperm motility, sperm morphology) and scrotal circumference in offspring. However, by evaluating associations between poor maternal gestational nutrition and altered small non-cording RNAs (sncRNAs) and DNA methylation in offspring sperm, we demonstrated that poor maternal gestational nutrition alters sperm sncRNA composition and expression. Whole genome bisulfite sequencing further identified genomic regions with increased or decreased DNA methylation in sperm in response to poor maternal gestational nutrition. These findings imply that maternal diet-induced epigenetic errors can accumulate in sperm to worsen developmental outcomes of future generations.
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| Overall design |
Comparative DNA CpG methylation of WGBS data of F1 ram sperm in CON, OVER and RES group.
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| Contributor(s) |
Zhu L, Scatolin G |
| Citation(s) |
37647207 |
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| Submission date |
Feb 23, 2023 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Zongliang Jiang |
| Organization name |
University of Florida
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| Department |
Animal Sciences
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| Street address |
2033 Mowry Rd
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
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| Samples (14)
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| GSM7060417 |
F1 ram sperm, CON, rep4 |
| GSM7060418 |
F1 ram sperm, OVER, rep1 |
| GSM7060419 |
F1 ram sperm, OVER, rep2 |
| GSM7060420 |
F1 ram sperm, OVER, rep3 |
| GSM7060421 |
F1 ram sperm, OVER, rep4 |
| GSM7060422 |
F1 ram sperm, OVER, rep5 |
| GSM7060423 |
F1 ram sperm, RES, rep1 |
| GSM7060424 |
F1 ram sperm, RES, rep2 |
| GSM7060425 |
F1 ram sperm, RES, rep3 |
| GSM7060426 |
F1 ram sperm, RES, rep4 |
| GSM7060427 |
F1 ram sperm, RES, rep5 |
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| This SubSeries is part of SuperSeries: |
| GSE225988 |
Maternal gestational nutrition perturbs offspring sperm epigenome in sheep |
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| Relations |
| BioProject |
PRJNA938069 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE225924_RAW.tar |
804.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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