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| Status |
Public on Mar 01, 2023 |
| Title |
'Effect of markerless deletion of PA14_16550 from P. aeruginosa PA14 biofilm colonies on M6301-glycerol agar for 3 d at 25C |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine the transcriptomic effect when PA14_16550, which encodes a TetR-type DNA-binding protein, is absent from cells. Deletion of this gene results in lower exopolysaccharide levels (a biofilm defect). Here, we compared PA14 deleted for amrZ (which has a moderately hyper-biofilm phenotype) and cells deleted for both amrZ and 16550. We also analyzed wild-type PA14 cells.
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| Overall design |
Total RNA was extracted from mechanically dispersed colonies grown on M6301-1% agar for 3 d at 25C. Transcriptomes were compared among PA14, PA14 delta-amrZ, and PA14 delta-amrZ delta-16550.
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| Contributor(s) |
Yahya AH, Cabeen MT, Pan S |
| Citation(s) |
36971546, 37754547 |
| |
| Submission date |
Feb 24, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Matthew Cabeen |
| E-mail(s) |
matthew.cabeen@okstate.edu
|
| Phone |
4057446652
|
| Organization name |
Oklahoma State University
|
| Department |
Microbiology and Molecular Genetics
|
| Street address |
307 Life Science East
|
| City |
Stillwater |
| State/province |
Oklahoma |
| ZIP/Postal code |
74078 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL26913 |
Illumina NovaSeq 6000 (Pseudomonas aeruginosa) |
|
| Samples (11)
|
| GSM7063834 |
PA14 delta-amrZ cells, biol rep 1 |
| GSM7063835 |
PA14 delta-amrZ cells, biol rep 2 |
| GSM7063836 |
PA14 delta-amrZ cells, biol rep 3 |
| GSM7063837 |
PA14 delta-amrZ cells, biol rep 4 |
| GSM7063838 |
PA14 delta-amrZ delta-16550 cells, biol rep 1 |
| GSM7063839 |
PA14 delta-amrZ delta-16550 cells, biol rep 2 |
| GSM7063840 |
PA14 delta-amrZ delta-16550 cells, biol rep 3 |
| GSM7063841 |
PA14 delta-amrZ delta-16550 cells, biol rep 4 |
|
| Relations |
| BioProject |
PRJNA938508 |
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