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| Status |
Public on Oct 16, 2024 |
| Title |
Targeting PELP1 reduces endometrial cancer progression via attenuation of ribosomal biogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We examined the mechanisms by which SMIP34 regulates EEC progression. EEC cells (HEC-1-A) were treated with either vehicle or SMIP34 20µM for 24 hours, and total RNA was isolated for RNA-seq analysis. Our results demonstrated that PELP1 inhibition by SMIP34 reduces the expression of genes involved in ribosome biogenesis and translation.
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| Overall design |
Total RNA from HEC-1-A control, and SMIP34-treated cells were isolated using RNeasy mini kit (Qiagen). For whole-genome transcriptome profiling, 2 libraries (3 replicates from each group) were generated using a TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s protocol (Illumina Inc.). Samples were sequenced on the Illumina HiSeq 3000 platform (Illumina Inc.) using the 50 base-pair single-read (50SR) sequencing module.
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| Contributor(s) |
Vadlamudi RK, Yang X, Chen Y, Lai Z |
| Citation(s) |
37853941 |
| |
| Submission date |
Mar 07, 2023 |
| Last update date |
Oct 17, 2024 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
|
| Phone |
2105629163
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA941881 |
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