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| Status |
Public on Aug 08, 2023 |
| Title |
An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria |
| Organism |
Synechococcus elongatus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Regulation of CO2 fixation in cyanobacteria is important both for the organism and the global carbon balance. Here we show that phosphoketolase in Synechococcus elongatus PCC7942 (SeXPK) possesses a distinct ATP sensing mechanism, which upon ATP drops, allows SeXPK to divert precursors of the RuBisCO substrate away from the Calvin-Benson-Bassham (CBB) cycle. Deleting the SeXPK gene increased CO2 fixation particularly during light-dark transitions. In high-density cultures, the xpk strain showed a 60% increase in carbon fixation, and unexpectedly resulted in sucrose secretion without any pathway engineering. Using cryo-EM analysis, we discovered that these functions were enabled by a unique allosteric regulatory site involving two subunits jointly binding two ATP, which constantly suppresses the activity of SeXPK until the ATP level drops. This magnesium-independent ATP allosteric site is present in many species across all three domains of life, where it may also play important regulatory functions.
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| Overall design |
Compare WT and del(xpk) transciptome of Synecococcus elongatus PCC7942 in different light conditions
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| Contributor(s) |
Lu K, Chang C, Wang C, Chen FY, Huang I, Huang P, Yang C, Wu H, Wu W, Hsu K, Ho M, Tsai M, Liao JC |
| Citation(s) |
37349485 |
| Submission date |
Mar 15, 2023 |
| Last update date |
Aug 09, 2023 |
| Contact name |
James C. Liao |
| E-mail(s) |
liaoj@gate.sinica.edu.tw
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| Organization name |
Academia Sinica
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| Street address |
128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan
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| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL33256 |
NextSeq 2000 (Synechococcus elongatus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA944967 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227397_RAW.tar |
2.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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