 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 30, 2024 |
| Title |
MYC promotes aggressive growth and metastasis of a WNT-medulloblastoma mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Medulloblastoma (MB), the most common malignant pediatric brain tumor, comprises of four molecularly and clinically distinct subgroups (termed WNT, SHH, Group3, and Group4). Prognosis varies based on genetic and pathological features associated with each molecular subgroup. WNT-MB, considered low-risk, are rarely metastatic and contain activating mutations in CTNNB1; Group3-MB, commonly classified as high-risk, are frequently metastatic and can contain genomic alterations resulting in elevated MYC expression. Here we compare model systems of low-risk WNT-MB to high-risk Group3-MB to identify tumor and microenvironment interactions that could contribute to features associated with poor outcome. Compared to Group3-MB, we find that WNT-MB display enrichment in gene sets related to extracellular matrix (ECM) regulation and cellular adhesion. Exogenous expression of MycT58A in murine WNT-MB models significantly accelerates growth and results in metastatic disease. In addition to down-regulation of ECM regulation and cell adhesion pathways, we also identified immune system interactions among the top down-regulated signaling pathways following MycT58A expression. Taken together, our data provides evidence that increased Myc signaling can promote the growth and metastasis of WNT-MB.
|
| |
|
| Overall design |
Three biological replicates were analyzed for each of 3 conditions (control cerebellum, mWnt-MB + EV, mWnt-MB + MycT58A)
|
| |
|
| Contributor(s) |
Phoenix T, Hartley R |
| Citation(s) |
37544301 |
| |
| Submission date |
Mar 17, 2023 |
| Last update date |
Aug 01, 2024 |
| Contact name |
Timothy Phoenix |
| E-mail(s) |
timothy.phoenix@uc.edu
|
| Phone |
5135587749
|
| Organization name |
University of Cincinnati
|
| Department |
Division of Pharmaceutical Sciences
|
| Lab |
Phoenix Lab
|
| Street address |
231 Albert Sabin Way, MSB 3005M
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA945935 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227631_Hartley_and_Phoenix_GEO_1_processed.txt.gz |
379.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...