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Series GSE228083 Query DataSets for GSE228083
Status Public on Aug 10, 2023
Title Clariom S Microarray Expression Data from Human Eosinophilic Esophagitis and Control Esophageal Biopsies
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study sought to evaluate the diffferential gene expression of human Eosinophilic Esophagitis (EoE) patients compared to control patients.
Abstract from associated publication: Eosinophilic Esophagitis (EoE) is a chronic allergic disease characterized by progressive inflammation of the esophageal mucosa. While the transcriptome of EoE has been reported, few studies have examined the genetics among both adult and pediatric EoE populations. Here, we use microarray analysis to interrogate gene expression using esophageal biopsies from EoE and Control subjects with a wide age distribution. Analysis of differential gene expression and prediction of impaired pathways was compared using conventional transcriptome analysis programs and an artificial intelligence-based ADVAITA program. Principal Components Analysis revealed samples cluster by disease status (EoE and Control) irrespective of clinical features like sex, age, and disease severity. Global transcriptomic analysis revealed differential expression of several genes previously reported in EoE (CCL26, CPA3, POSTN, CTSC, ANO1, CRISP3, SPINK7). In addition, we identified differential expression of several genes from the MUC and SPRR families, which has not been previously reported. Our findings suggest their involvement in impaired barrier integrity and loss of epidermal cell differentiation in EoE patients. These findings present two new gene families that are differentially expressed in EoE patients in both adult and pediatric populations, which presents an opportunity for a future therapeutic targets that would be useful in a large demographic of patients.
 
Overall design Biopsies were collected from endoscopically visible areas of active inflammation and healthy mucosa in subjects and controls, respectively. RNA extractions were collected from each biopsy using a Qiagen AllPrep Kit. Groups include Eosinophilic Esophagitis (EoE) (n=12) and control biopsies (n=12), totaling 24 samples. 1 HeLa MRSL, provided by the manufacturer, was included.
 
Contributor(s) Morrison HA, Allen IC
Citation(s) 37692891
Submission date Mar 23, 2023
Last update date Sep 15, 2023
Contact name Irving Coy Allen
E-mail(s) icallen@vt.edu
Organization name Virginia-Maryland College of Veterinary Medicine
Department Biomedical Sciences and Pathobiology
Street address 205 Duck Pond Dr.
City Blacksburg
State/province VA
ZIP/Postal code 24061
Country USA
 
Platforms (1)
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (25)
GSM7113628 E51
GSM7113629 E60
GSM7113630 E139
Relations
BioProject PRJNA948015

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228083.txt.gz 1.4 Kb (ftp)(http) TXT
GSE228083_RAW.tar 33.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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