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| Status |
Public on Apr 01, 2012 |
| Title |
Dynamic gene expression of two Caenorhabditis elegans wild type strains (N2 and CB4856) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
We analyzed the consequences of recombination in gene expression. For this, we measured expression patterns in two C. elegans wild types, N2 and CB4856 (CB). We compare these profiles with gene expression values from a Recombinant Inbred Population (RIL) derived from the same wild types. Expression values of the RILs (GSE17071) were obtained from Viñuela et al. (Genome Research, 2010). N2 and CB strain nematodes were cultured in identical conditions. Likewise, mRNA was extracted at three different ages (juveniles, reproductive and old worms) to investigate the transcriptional consequences of recombination in aging worms. Then, we explored gene expression heritability and transgression as genetic parameters for the analysis of gene expression divergence in natural isolates. Moreover, we investigated the progression of those parameters with age.
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| Overall design |
In total, 14 dual-color microarrays were done on two wild type strains (N2 and CB4856) and three age groups (40, 96, and 214 hours). 5 replicates of t1 (juvenile) and t3 (senescent) samples, and 4 replicates of t2 (reproductive) samples, in a dye-swap design.
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| Contributor(s) |
Viñuela A, Kammenga JE, Snoek LB |
| Citation(s) |
22670229 |
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| Submission date |
Jul 12, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Ana Viñuela |
| E-mail(s) |
ana.vinuela_rodriguez@kcl.ac.uk
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| Phone |
+44 (0) 20 7188 1505
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| URL |
http://www.anavinuela.com
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| Organization name |
King's College London
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| Department |
Twin Research & Genetic Epidemiology
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| Street address |
St.Thomas' Hospital Campus Westminster Bridge Road
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| City |
London |
| State/province |
London |
| ZIP/Postal code |
SE1 7EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL4038 |
C. elegans 22k 60 mer array |
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| Samples (14)
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| GSM565394 |
CB4856 vs. N2, T1, rep2 |
| GSM565395 |
N2 vs. CB4856, T1, rep3 |
| GSM565396 |
N2 vs. CB4856, T2, rep1 |
| GSM565397 |
CB4856 vs. N2, T2, rep1 |
| GSM565398 |
N2 vs. CB4856, T2, rep2 |
| GSM565399 |
N2 vs. CB4856, T2, rep3 |
| GSM565400 |
N2 vs. CB4856, T3, rep1 |
| GSM565401 |
N2 vs. CB4856, T3, rep2 |
| GSM565402 |
CB4856 vs. N2, T3, rep1 |
| GSM565403 |
N2 vs. CB4856, T3, rep3 |
| GSM565404 |
CB4856 vs. N2, T3, rep2 |
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| Relations |
| BioProject |
PRJNA128147 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22887_RAW.tar |
32.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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