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| Status |
Public on Dec 31, 2023 |
| Title |
Gene expression profile at single cell level from untreated and Enterococcus faecalis-infected skin wounds |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Enterococcus faecalis (E. faecalis) is a Gram-(+) opportunistic pathogen associated with predominantly nosocomial wound infections. E. faecalis has been shown to suppress or evade immune-mediated clearance by the immune system and promote persistent infection. Here, we sought to interrogate whether E. faecalis infection induces transcriptomic changes in the host at the single-cell resolution using a mouse excisional wound model. Keratinocyte, fibroblast, endothelial and immune cell populations reveal unique clusters in the infected wounds. Cell communication analysis discovered strong ligand-receptor interactions between macrophages and endothelial cells in the infected niche, whilst fibroblast and keratinocytes instruct healing cellular interactions in untreated skin wounds. We also identified differential terminal lineage driving genes, following RNA velocity in each condition. Together, results suggest that E. faecalis infection alters the skin transcriptome, which may help promote the chronicity of bacteria-infected wounds.
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| Overall design |
A single, 6-mm full-thickness wound was created on the shaven dorsal skin of C57BL/6J mice. The wounds were inoculated with Enterococcus faecalis and all wounds were covered with Tegaderm. Tissues were harvested at day 4 with a 6-mm biopys punch with minimal adjacent healthy tissue.
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| Web link |
http://doi.org/10.7554/eLife.95113
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| |
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| Contributor(s) |
Cenk C, Guillaume T |
| Citation(s) |
38767331 |
| |
| Submission date |
Apr 08, 2023 |
| Last update date |
May 21, 2024 |
| Contact name |
Guillaume Thibault |
| E-mail(s) |
thibaultlabo@gmail.com
|
| Organization name |
Nanyang Technological University
|
| Department |
School of Biological Sciences
|
| Lab |
Thibault Lab
|
| Street address |
60 Nanyang Dr
|
| City |
Singapore |
| ZIP/Postal code |
637551 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
| GSM7157726 |
Uninfected wound from dorsal skin replicate 1 |
| GSM7157727 |
Uninfected wound from dorsal skin replicate 2 |
| GSM7157728 |
E. faecalis infected wound from dorsal skin replicate 1 |
| GSM7157729 |
E. faecalis infected wound from dorsal skin replicate 2 |
|
| Relations |
| BioProject |
PRJNA953657 |
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