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| Status |
Public on Sep 05, 2023 |
| Title |
Gene expression profile of canine Chimeric Antigen Receptor (CAR) Engineered invariant Natural Killer T (iNKT) cells |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by array
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| Summary |
We used Nanostring gene expression profiling to characterize CAR-engineered canine iNKT cells
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| Overall design |
The anti-IL13Rα2-28-ζ CAR construct was expressed along with a truncated canine CD19 marker. End-of-expansion, resting CAR-iNKTs were stimulated for 4-8 hours with anti-CD3/CD28-coated beads at 1:1 ratio. RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions. Gene expression profiling was performed using NanoString Canine Immuno-Oncology panel and nCounter instrument (Wistar Institute) according to the manufacturer’s instructions.
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| Contributor(s) |
Rotolo A, Whelan EC, Atherton MJ, Kulikovskaya I, Jarocha D, Fraietta JA, Kim MM, Diffenderfer ES, Cengel KA, Piviani M, Radaelli E, Duran-Struuck R, Mason NJ |
| Citation(s) |
37852175 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 CA252619 |
Prognostic and Therapeutic Implications of IFNAR1 Signaling on CAR T Cell Therapy for Cancer |
UNIVERSITY OF PENNSYLVANIA |
Matthew John Atherton |
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| Submission date |
Apr 12, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Antonia Rotolo |
| E-mail(s) |
antonia.rotolo@pennmedicine.upenn.edu
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| Phone |
2674696507
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| Organization name |
University of Pennsylvania
|
| Street address |
3900 Chestnut Street, Apt 521
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL32796 |
Canine IO NanoString Probeset [gene name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA954687 |
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