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| Status |
Public on Mar 05, 2024 |
| Title |
The Alk receptor tyrosine kinase regulates Sparkly, a novel activity regulating neuropeptide precursor in the Drosophila CNS |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila, including functions in the CNS, however the molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of Alk signaling in the larval CNS. TaDa was employed in larval CNS tissues, while genetically manipulating Alk signaling output. The resulting TaDa data were analysed together with larval CNS scRNA-seq datasets performed under similar conditions, identifying a role for Alk in the transcriptional regulation of neuroendocrine gene expression. Further integration with bulk/scRNA-seq and protein datasets from larval brains in which Alk signaling was manipulated, identified a previously uncharacterized Drosophila neuropeptide precursor encoded by CG4577 as an Alk signaling transcriptional target. CG4577, which we named Sparkly (Spar), is expressed in a subset of Alk-positive neuroendocrine cells in the developing larval CNS, including circadian clock neurons. In agreement with our TaDa analysis, overexpression of the Drosophila Alk ligand Jeb resulted in increased levels of Spar protein in the larval CNS. We show that Spar protein is expressed in circadian (Clock) neurons, and Spar mutants exhibit defects in sleep and circadian rhythm control. In summary, we report a novel activity regulating neuropeptide precursor gene that is regulated by Alk signaling in the Drosophila CNS.
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| Overall design |
Targeted DamID (TaDa) transcriptional profiling to identify transcriptional targets of Alk signaling in the larval CNS.
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| Web link |
https://elifesciences.org/reviewed-preprints/88985v2
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| Contributor(s) |
Palmer R, Anthonydhason V, Sukumar SK |
| Citation missing |
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| Submission date |
Apr 12, 2023 |
| Last update date |
Mar 05, 2024 |
| Contact name |
Vimala Anthonydhason |
| Organization name |
University of Gothenburg
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| Department |
Institute of Biomedicine
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| Street address |
Medicinaregatan 9 a-b
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| City |
Göteborg |
| ZIP/Postal code |
41390 |
| Country |
Sweden |
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| Platforms (1) |
| GPL23702 |
HiSeq X Ten (Drosophila melanogaster) |
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| Samples (10)
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| GSM7164770 |
Dam-PolII expression with C155-Gal4 promoter Ctrl 1 replicate 1 |
| GSM7164771 |
Dam-PolII expression with C155-Gal4 promoter Ctrl 1 replicate 2 |
| GSM7164772 |
Dam-PolII expression with C155-Gal4 promoter Ctrl 2 replicate 1 |
| GSM7164773 |
Dam-PolII expression with C155-Gal4 promoter Ctrl 2 replicate 2 |
| GSM7164774 |
Dam-PolII expression with C155-Gal4 promoter Ctrl 3 replicate 1 |
| GSM7164775 |
Dam-PolII expression with C155-Gal4 promoter Ctrl 3 replicate 2 |
| GSM7164776 |
UAS-Alk.DN, Alk inhibition with C155-Gal4 promoter Neg 1 replicate 1 |
| GSM7164777 |
UAS-Alk.DN, Alk inhibition with C155-Gal4 promoter Neg 1 replicate 2 |
| GSM7164778 |
UAS-Alk.DN, Alk inhibition with C155-Gal4 promoter Neg 2 replicate 1 |
| GSM7164779 |
UAS-Alk.DN, Alk inhibition with C155-Gal4 promoter Neg 2 replicate 2 |
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| Relations |
| BioProject |
PRJNA954825 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE229518_Ctrl_vs_Domnegative_GATC.bw |
4.3 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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