|
| Status |
Public on Aug 23, 2010 |
| Title |
mRNA expression in wild-type of Thermus thermophilus HB8 strain grown on a rich (TT) medium at 45˚C and 70˚C |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
|
| Summary |
We observed the expression profile of the total mRNA of wild-type of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 45˚C compared with that of 70˚C.
Keywords: Growth temperature
|
| |
|
| Overall design |
Wild-type T. thermophilus HB8 strain was pre-cultured at 70˚C for 5 h in 5 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.5 with NaOH. The cells (5 ml) were inoculated into 0.30 liter of the same medium and then cultivated at 70˚C. At the Abs600 =0.8 (0min), this culture was divided into two flasks. Then, equal volume of cold medium (4˚C) was added and the temperature were shifted down to 45˚C. Hot medium (70˚C) was added to the other flask. Cells were collected at 0.5 min and 10 min after temperature shift in the equal volume of 100% ice-cold ethanol, and then crude RNA was extracted. In order to compare mRNA expression in the wild-type strain between 45˚C and 70˚C, the expression of each mRNA at each time point was analyzed on a GeneChip as described for each sample in this website.
|
| |
|
| Contributor(s) |
Mega R, Shinkai A, Nakagawa N, Masui R, Kuramitsu S |
| Citation(s) |
20655297, 21531768 |
| |
| Submission date |
Jul 15, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Ryosuke Mega |
| E-mail(s) |
xmega@bio.sci.osaka-u.ac.jp
|
| Phone |
+81-6-6850-5434
|
| Fax |
+81-6-6850-5442
|
| URL |
http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/kuramitu/index.html
|
| Organization name |
Osaka University
|
| Department |
Biological Sciences
|
| Lab |
Structural and Functional Analyses on Biomolecules
|
| Street address |
1-1 Machikaneyama-cho
|
| City |
Toyonaka |
| State/province |
Osaka |
| ZIP/Postal code |
560-0043 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL4902 |
Riken Thermus thermophilus HB8 4K mRNA array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA127849 |
Less...
More...