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| Status |
Public on May 17, 2024 |
| Title |
Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3 [Affymetrix] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Epithelial cells are the first point of contact for bacteria entering the respiratory tract. Streptococcus pneumoniae is an obligatory human pathobiont of the nasal mucosa, carried asymptomatically but also the cause of severe pneumoniae. The role of the epithelium in maintaining homeostatic interactions or mounting an inflammatory response to invasive S. pneumoniae is currently poorly understood. However, studies have shown that chromatin modifications, at the histone level, induced by bacterial pathogens interfere with the host transcriptional program and promote infection. In this study, we demonstrate that S. pneumoniae actively induces di-methylation of histone H3 on lysine 4 (H3K4me2), which persists for at least 9 days upon clearance of bacteria with antibiotics. We show that infection establishes a unique epigenetic program affecting the transcriptional response of epithelial cells, rendering them more permissive upon secondary infection. Our results establish H3K4me2 as a unique modification induced by infection, distinct from H3K4me3, which localizes to enhancer regions genome-wide. Therefore, this study reveals evidence that bacterial infection leaves a memory in epithelial cells after bacterial clearance, in an epigenomic mark, thereby altering cellular responses for subsequent infections.
Transcriptome analysis aimed at testing whether cells respond differently in the primary (1°) and secondary (2°) infection. Differential gene expression and gene set enrichment analysis performed.
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| Overall design |
Microarrays are used to measure gene expression along an infection time course: uninfected (UI), primary infection (1°) and post-infection (PI), 3 replicates per condition.
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| Contributor(s) |
Chevalier C, Chica C, Pain A, Hamon M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 20, 2023 |
| Last update date |
May 18, 2024 |
| Contact name |
Claudia Chica |
| Organization name |
Institut Pasteur
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| Lab |
Biostatistics and Bioinformatics Hub
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| Street address |
25-28 Rue du Dr Roux
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| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platforms (1) |
| GPL17692 |
[HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM7188072 |
A549 cells, primary, REP1 |
| GSM7188073 |
A549 cells, primary, REP2 |
| GSM7188074 |
A549 cells, primary, REP3 |
| GSM7188075 |
A549 cells, PI, REP1 |
| GSM7188076 |
A549 cells, PI, REP2 |
| GSM7188077 |
A549 cells, PI, REP3 |
| GSM7188078 |
A549 cells, secondary, REP1 |
| GSM7188079 |
A549 cells, secondary, REP2 |
| GSM7188080 |
A549 cells, secondary, REP3 |
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| This SubSeries is part of SuperSeries: |
| GSE230142 |
Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3 |
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| Relations |
| BioProject |
PRJNA957721 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230138_RAW.tar |
51.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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