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| Status |
Public on Jul 05, 2023 |
| Title |
AoMedA has complex regulatory relationship with AoBrlA, AoAbaA and AoWetA in conidiation, trap formation, and secondary metabolism in nematode-trapping fungus Arthrobotrys oligospora |
| Organism |
Orbilia oligospora |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptomic analysis revealed that differentially expressed genes in the absence of AomedA and AoWetA were involved in mycelial growth, development process,conidiation, cell nucleus, trap formation, peroxisome, endocytosis and lipid metabolism.
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| Overall design |
WT (represented by AO and AOB) and ΔAomedA (represented by MedA) and ΔAowetA (represented by WetA) strains grown on cellophane-overlaid CMY plates at 28°C for 5 days, three replicates were used for each sample. After collecting the samples, RNA was extracted with the AxyPrep Multisource RNA Miniprep Kit (Axygen, Jiangsu, China), and then sent to Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) to construct the transcriptome of WT and ΔAomedA and ΔAowetA strains. The final cDNA library was sequenced on an Illumina Novaseq 4000 platform.
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| Web link |
http://doi: 10.1128/aem.00983-23.
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| Contributor(s) |
Bai N, Xie M, Liu Q, Zhu Y, Yang X, Zhang K, Yang J |
| Citation(s) |
37655869 |
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| Submission date |
Apr 21, 2023 |
| Last update date |
Oct 10, 2023 |
| Contact name |
na Bai |
| E-mail(s) |
baina@mail.ynu.edu.cn
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| Phone |
18468176193
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| Organization name |
Yunnan University
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| Street address |
No. 2, Cuihu North Road
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| City |
Kunming |
| ZIP/Postal code |
650091 |
| Country |
China |
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| Platforms (1) |
| GPL31134 |
Illumina HiSeq 4000 (Orbilia oligospora) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA958210 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230299_Comparison_result_statistics_table.csv.gz |
500 b |
(ftp)(http) |
CSV |
| GSE230299_Expression_analysis_table_include_TPM_values_for_each_sample.csv.gz |
505.4 Kb |
(ftp)(http) |
CSV |
| GSE230299_Matrix_table_with_raw_gene_counts_for_every_gene_and_every_sample.csv.gz |
497 b |
(ftp)(http) |
CSV |
| GSE230299_Sequencing_data_statistics_of_transcriptome.csv.gz |
582 b |
(ftp)(http) |
CSV |
| GSE230299_Software_Information_Table.csv.gz |
1.8 Kb |
(ftp)(http) |
CSV |
| GSE230299_Statistical_table_of_difference_in_expression.csv.gz |
30.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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