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| Status |
Public on Apr 23, 2023 |
| Title |
RNAseq of dauer-forming C. elegans L2d in daf-2 and daf-2;daf-42 |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Under adverse environmental conditions, nematodes arrest into dauer, an alternative developmental stage for diapause. Dauers endure unfavorable environment and interact with host animals to access favorable environments, thus playing a critical role in the survival of both free-living and parasitic nematodes. Here, we discovered that in Caenorhabditis elegans, daf-42 is essential for development into dauer stage, as the null mutant shows lethal phenotype during dauer entry. To examine the transcriptional changes accompanied by the absence of DAF-42 during dauer entry, we performed RNA-sequencing on daf-2 and daf-2; daf-42 worms at 52 hours after egg laying (HAE) and 60 HAE, the time at which 0% and about 40% of daf-2; daf-42 mutants form dead dauer at 25 degrees Celcius, respectively. daf-2 control worms develop into dauer stage after 60 HAE, and half of its population develop into dauer by 72 HAE, when raised at 25 degrees Celcius.
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| Overall design |
daf-2(e1370) and daf-2(e1370);daf-42(ys54) C.elegans at late L2d stages, at 52 and 60 hours after eggs were laid, raised at 25°C
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| Contributor(s) |
Lim DS, Kim J, Lee J |
| Citation(s) |
37216205 |
| BioProject |
PRJNA871468 |
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| Submission date |
Apr 22, 2023 |
| Last update date |
Jul 23, 2023 |
| Contact name |
Daisy Sunghee Lim |
| E-mail(s) |
daisylim@snu.ac.kr
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| Organization name |
Seoul National University
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| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
| GPL26672 |
Illumina NovaSeq 6000 (Caenorhabditis elegans) |
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| Samples (12)
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| GSM7220290 |
daf-2, 52 Hours after eggs laid, replicate 1 |
| GSM7220291 |
daf-2, 52 Hours after eggs laid, replicate 2 |
| GSM7220292 |
daf-2, 52 Hours after eggs laid, replicate 3 |
| GSM7220293 |
daf-2; daf-42, 52 Hours after eggs laid, replicate 1 |
| GSM7220294 |
daf-2; daf-42, 52 Hours after eggs laid, replicate 2 |
| GSM7220295 |
daf-2; daf-42, 52 Hours after eggs laid, replicate 3 |
| GSM7220296 |
daf-2, 60 Hours after eggs laid, replicate 1 |
| GSM7220297 |
daf-2, 60 Hours after eggs laid, replicate 2 |
| GSM7220298 |
daf-2, 60 Hours after eggs laid, replicate 3 |
| GSM7220299 |
daf-2; daf-42, 60 Hours after eggs laid, replicate 1 |
| GSM7220300 |
daf-2; daf-42, 60 Hours after eggs laid, replicate 2 |
| GSM7220301 |
daf-2; daf-42, 60 Hours after eggs laid, replicate 3 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE230353_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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