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| Status |
Public on Jun 05, 2024 |
| Title |
NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days |
| Organisms |
Haemophilus influenzae; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days
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| Overall design |
Normal human nasal epithelia (NHNE) from five healthy human donors were infected with Haemophilus influenzae strain 86-028NP (MOI 1:100, NHNE:bacteria). Samples for RNA isolation were collected prior to infection (day0) for uninfected NHNE and bacterial pre-cultures, and on day1, 3, 7 and 14 post-infection for NTHi-infected NHNE. RNA was isolated using TRIZOL, treated with EZDnase to remove remaining gDNA and concentrated usingRNA Clean and Concentrate Kits (Zymo). 2-3 microg total RNA (RIN >8.0) were ribodepleted using the RiboMinus kit (Termo Fisher) and the ribodepleted RNA used for library preparation. For all NHNE samples ~ 70 million reads were collected per sample and mapped to human and NTHi reference genomes. for NTHi infected NHNE samples, approximately 2 milion reads could be mapped to the bacterial genome.
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| Contributor(s) |
Kappler U, Fantino E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 23, 2023 |
| Last update date |
Jun 05, 2024 |
| Contact name |
Ulrike Kappler |
| E-mail(s) |
u.kappler@uq.edu.au
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| Phone |
+61 7 33652978
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| Organization name |
The University of Queensland
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| Department |
School of Chemistry and Molecular Biosciences
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| Street address |
Cooper Rd B76
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| City |
St. Lucia |
| State/province |
Qld |
| ZIP/Postal code |
4072 |
| Country |
Australia |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25730 |
Illumina NovaSeq 6000 (Haemophilus influenzae) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA960846 |
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