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| Status |
Public on May 16, 2024 |
| Title |
Investigation of transcript 3' ends in Campylobacter jejuni wildtype and ribonuclease mutants |
| Organism |
Campylobacter jejuni |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Termination site sequencing (term-seq) (Dar et al., 2016) was used to map transcript 3' ends and potential processing sites in Campylobacter jejuni NCTC11168 wildtype and ribonuclease deletion strains.
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| Overall design |
Total RNA was extracted from C. jejuni NCTC11168 WT and RNase III (rnc, Cj1635c), RNase Y (rny, Cj1209), PNPase (pnp, Cj1253), and RNase R (rnr, Cj0631c) deletion strains grown to exponential phase, genomic DNA was removed with DNase I and rRNA depleted, cDNA libraries were prepared at Vertis Biotechnologie AG, Freising, Germany, deep-sequencing was performed on an Illumina NextSeq 500 instrument
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| Contributor(s) |
König F, Svensson SL, Sharma CM |
| Citation(s) |
38897989 |
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| Submission date |
Apr 28, 2023 |
| Last update date |
Jun 20, 2024 |
| Contact name |
Cynthia Sharma |
| Organization name |
University of Würzburg
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| Street address |
Josef-Schneider-Straße 2
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| City |
Würzburg |
| State/province |
Bavaria |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (1) |
| GPL26884 |
Illumina NextSeq 500 (Campylobacter jejuni) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA962813 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230837_RAW.tar |
160.6 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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