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| Status |
Public on May 01, 2023 |
| Title |
Chronic hypercapnia-induced gene expression profiles in the brain |
| Organism |
Capra hircus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To test the hypothesis that increasing severity of chronic hypercapnia elicits distint gene expression profiles in important brainstem regions of cardiorespiratory control, three groups of goats (female; n=6/group) were exposed either 14 days of room air (RA), 14 days of 6% inspired CO2 (mild CH), or 7d of 6% InCO2 followed by 7d of 8% InCO2 (moderate CH). Following 14 days of exposure 4 target sites were collected (3 brainstem/1 cortical) for gene expression profiling analysis using bulk-tissue RNA-sequencing. Comparisons were made across all target sites comparing room air animals to either mild or moderate CH.
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| Overall design |
Comparative gene expression analysis of RNA-seq data from 4 brain regions (mPFC, NTS, VRC, RTN) collected from animals (n=6/condition) exposed one of 3 different conditions (normal room air,mild CH, or moderate CH). Comparisons were made for RA vs. Mild or RA vs. Moderate CH across all 4 brain regions.
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| Contributor(s) |
Grams K, Mathison A, Zimmerman M, Miller A, Abrudan J, Wendt Andrae J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 29, 2023 |
| Last update date |
May 01, 2023 |
| Contact name |
Kirstyn J Grams |
| E-mail(s) |
kgrams@mcw.edu
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| Organization name |
Medical College of Wisconsin
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| Department |
Physiology
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| Lab |
Forster
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| Street address |
8701 W Watertown Plank Road
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| City |
Milwaukee |
| State/province |
Wisconsin |
| ZIP/Postal code |
53226 |
| Country |
USA |
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| Platforms (1) |
| GPL27106 |
Illumina NovaSeq 6000 (Capra hircus) |
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| Samples (72)
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| GSM7256818 |
Medial Prefrontal Cortex, 14 days, Room Air, Replicate 1 |
| GSM7256819 |
Medial Prefrontal Cortex, 14 days, Room Air, Replicate 2 |
| GSM7256820 |
Medial Prefrontal Cortex, 14 days, Room Air, Replicate 3 |
| GSM7256821 |
Medial Prefrontal Cortex, 14 days, Room Air, Replicate 4 |
| GSM7256822 |
Medial Prefrontal Cortex, 14 days, Room Air, Replicate 5 |
| GSM7256823 |
Medial Prefrontal Cortex, 14 days, Room Air, Replicate 6 |
| GSM7256824 |
Medial Prefrontal Cortex, 14 days, Mild CH, Replicate 1 |
| GSM7256825 |
Medial Prefrontal Cortex, 14 days, Mild CH, Replicate 2 |
| GSM7256826 |
Medial Prefrontal Cortex, 14 days, Mild CH, Replicate 3 |
| GSM7256827 |
Medial Prefrontal Cortex, 14 days, Mild CH, Replicate 4 |
| GSM7256828 |
Medial Prefrontal Cortex, 14 days, Mild CH, Replicate 5 |
| GSM7256829 |
Medial Prefrontal Cortex, 14 days, Mild CH, Replicate 6 |
| GSM7256830 |
Medial Prefrontal Cortex, 7 days 6%-7 days 8%, Moderate CH, Replicate 1 |
| GSM7256831 |
Medial Prefrontal Cortex, 7 days 6%-7 days 8%, Moderate CH, Replicate 2 |
| GSM7256832 |
Medial Prefrontal Cortex, 7 days 6%-7 days 8%, Moderate CH, Replicate 3 |
| GSM7256833 |
Medial Prefrontal Cortex, 7 days 6%-7 days 8%, Moderate CH, Replicate 4 |
| GSM7256834 |
Medial Prefrontal Cortex, 7 days 6%-7 days 8%, Moderate CH, Replicate 5 |
| GSM7256835 |
Medial Prefrontal Cortex, 7 days 6%-7 days 8%, Moderate CH, Replicate 6 |
| GSM7256836 |
Nucleus Tractus Solitarii, 14 days, Room Air, Replicate 1 |
| GSM7256837 |
Nucleus Tractus Solitarii, 14 days, Room Air, Replicate 2 |
| GSM7256838 |
Nucleus Tractus Solitarii, 14 days, Room Air, Replicate 3 |
| GSM7256839 |
Nucleus Tractus Solitarii, 14 days, Room Air, Replicate 4 |
| GSM7256840 |
Nucleus Tractus Solitarii, 14 days, Room Air, Replicate 5 |
| GSM7256841 |
Nucleus Tractus Solitarii, 14 days, Room Air, Replicate 6 |
| GSM7256842 |
Nucleus Tractus Solitarii, 14 days, Mild CH, Replicate 1 |
| GSM7256843 |
Nucleus Tractus Solitarii, 14 days, Mild CH, Replicate 2 |
| GSM7256844 |
Nucleus Tractus Solitarii, 14 days, Mild CH, Replicate 3 |
| GSM7256845 |
Nucleus Tractus Solitarii, 14 days, Mild CH, Replicate 4 |
| GSM7256846 |
Nucleus Tractus Solitarii, 14 days, Mild CH, Replicate 5 |
| GSM7256847 |
Nucleus Tractus Solitarii, 14 days, Mild CH, Replicate 6 |
| GSM7256848 |
Nucleus Tractus Solitarii, 7 days 6%-7 days 8%, Moderate CH, Replicate 1 |
| GSM7256849 |
Nucleus Tractus Solitarii, 7 days 6%-7 days 8%, Moderate CH, Replicate 2 |
| GSM7256850 |
Nucleus Tractus Solitarii, 7 days 6%-7 days 8%, Moderate CH, Replicate 3 |
| GSM7256851 |
Nucleus Tractus Solitarii, 7 days 6%-7 days 8%, Moderate CH, Replicate 4 |
| GSM7256852 |
Nucleus Tractus Solitarii, 7 days 6%-7 days 8%, Moderate CH, Replicate 5 |
| GSM7256853 |
Nucleus Tractus Solitarii, 7 days 6%-7 days 8%, Moderate CH, Replicate 6 |
| GSM7256854 |
Retrotrapezoid Nucleus, 14 days, Room Air, Replicate 1 |
| GSM7256855 |
Retrotrapezoid Nucleus, 14 days, Room Air, Replicate 2 |
| GSM7256856 |
Retrotrapezoid Nucleus, 14 days, Room Air, Replicate 3 |
| GSM7256857 |
Retrotrapezoid Nucleus, 14 days, Room Air, Replicate 4 |
| GSM7256858 |
Retrotrapezoid Nucleus, 14 days, Room Air, Replicate 5 |
| GSM7256859 |
Retrotrapezoid Nucleus, 14 days, Room Air, Replicate 6 |
| GSM7256860 |
Retrotrapezoid Nucleus, 14 days, Mild CH, Replicate 1 |
| GSM7256861 |
Retrotrapezoid Nucleus, 14 days, Mild CH, Replicate 2 |
| GSM7256862 |
Retrotrapezoid Nucleus, 14 days, Mild CH, Replicate 3 |
| GSM7256863 |
Retrotrapezoid Nucleus, 14 days, Mild CH, Replicate 4 |
| GSM7256864 |
Retrotrapezoid Nucleus, 14 days, Mild CH, Replicate 5 |
| GSM7256865 |
Retrotrapezoid Nucleus, 14 days, Mild CH, Replicate 6 |
| GSM7256866 |
Retrotrapezoid Nucleus, 7 days 6%-7 days 8%, Moderate CH, Replicate 1 |
| GSM7256867 |
Retrotrapezoid Nucleus, 7 days 6%-7 days 8%, Moderate CH, Replicate 2 |
| GSM7256868 |
Retrotrapezoid Nucleus, 7 days 6%-7 days 8%, Moderate CH, Replicate 3 |
| GSM7256869 |
Retrotrapezoid Nucleus, 7 days 6%-7 days 8%, Moderate CH, Replicate 4 |
| GSM7256870 |
Retrotrapezoid Nucleus, 7 days 6%-7 days 8%, Moderate CH, Replicate 5 |
| GSM7256871 |
Retrotrapezoid Nucleus, 7 days 6%-7 days 8%, Moderate CH, Replicate 6 |
| GSM7256872 |
Ventral Respiratory Column, 14 days, Room Air, Replicate 1 |
| GSM7256873 |
Ventral Respiratory Column, 14 days, Room Air, Replicate 2 |
| GSM7256874 |
Ventral Respiratory Column, 14 days, Room Air, Replicate 3 |
| GSM7256875 |
Ventral Respiratory Column, 14 days, Room Air, Replicate 4 |
| GSM7256876 |
Ventral Respiratory Column, 14 days, Room Air, Replicate 5 |
| GSM7256877 |
Ventral Respiratory Column, 14 days, Room Air, Replicate 6 |
| GSM7256878 |
Ventral Respiratory Column, 14 days, Mild CH, Replicate 1 |
| GSM7256879 |
Ventral Respiratory Column, 14 days, Mild CH, Replicate 2 |
| GSM7256880 |
Ventral Respiratory Column, 14 days, Mild CH, Replicate 3 |
| GSM7256881 |
Ventral Respiratory Column, 14 days, Mild CH, Replicate 4 |
| GSM7256882 |
Ventral Respiratory Column, 14 days, Mild CH, Replicate 5 |
| GSM7256883 |
Ventral Respiratory Column, 14 days, Mild CH, Replicate 6 |
| GSM7256884 |
Ventral Respiratory Column, 7 days 6%-7 days 8%, Moderate CH, Replicate 1 |
| GSM7256885 |
Ventral Respiratory Column, 7 days 6%-7 days 8%, Moderate CH, Replicate 2 |
| GSM7256886 |
Ventral Respiratory Column, 7 days 6%-7 days 8%, Moderate CH, Replicate 3 |
| GSM7256887 |
Ventral Respiratory Column, 7 days 6%-7 days 8%, Moderate CH, Replicate 4 |
| GSM7256888 |
Ventral Respiratory Column, 7 days 6%-7 days 8%, Moderate CH, Replicate 5 |
| GSM7256889 |
Ventral Respiratory Column, 7 days 6%-7 days 8%, Moderate CH, Replicate 6 |
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| Relations |
| BioProject |
PRJNA963193 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231308_DEAnalyses_pairwise_EdgeR.xlsx |
42.3 Mb |
(ftp)(http) |
XLSX |
| GSE231308_GeneCounts_raw_RPKM.csv.gz |
9.6 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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