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| Status |
Public on May 15, 2023 |
| Title |
IgE-binding monocytes upregulate the coagulation cascade in allergic horses |
| Organism |
Equus caballus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
IgE-binding monocytes are a rare peripheral immune cell type involved in the allergic response through binding of IgE on their surface. IgE-binding monocytes are present in both healthy and allergic individuals. We performed RNA sequencing to ask how the function of IgE-binding monocytes differs in the context of allergy. Using a large animal model of allergy, equine Culicoides hypersensitivity, we compared the transcriptome of IgE-binding monocytes in allergic and non-allergic horses at two seasonal timepoints: (i) when allergic animals were clinical healthy, in the winter “Remission Phase”, and (ii) during chronic disease, in the summer “Clinical Phase”. Most transcriptional differences between allergic and non-allergic horses occurred only during the “Remission Phase”, suggesting principal differences in monocyte function even in the absence of allergen exposure. F13A1, a subunit of fibrinoligase, was significantly upregulated at both timepoints in allergic horses. This suggested a role for increased fibrin deposition in the coagulation cascade to promote allergic inflammation. IgE-binding monocytes also downregulated CCR10 expression in allergic horses during the “Clinical Phase”, suggesting a defect in maintenance of skin homeostasis, which further promotes allergic inflammation. Together, this transcriptional analysis provides valuable clues into the mechanisms used by IgE-binding monocytes in allergic individuals.
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| Overall design |
Flow cytometry isolated IgE-binding monocytes were purified from equine peripheral blood. Monocytes were purified from allergic (n=7) and healthy (n=5) horses during allergen exposure (Clinical Phase in August-September) and when there was no allergen exposure (Remission Phase in January-February).
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| Contributor(s) |
Simonin EM, Wagner B |
| Citation(s) |
37193769 |
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| Submission date |
May 01, 2023 |
| Last update date |
Aug 18, 2023 |
| Contact name |
Bettina Wagner |
| E-mail(s) |
bw73@cornell.edu
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| Organization name |
Cornell University
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| Department |
Population Medicine and Diagnostic Sciences
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| Street address |
240 Farrier Drive
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14815 |
| Country |
USA |
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| Platforms (1) |
| GPL21401 |
Illumina NextSeq 500 (Equus caballus) |
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| Samples (24)
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| GSM7277543 |
IgE+Monocytes, Allergic, Remission Phase EL1 |
| GSM7277544 |
IgE+Monocytes, Allergic, Remission Phase EL2 |
| GSM7277545 |
IgE+Monocytes, Allergic, Remission Phase EL3 |
| GSM7277546 |
IgE+Monocytes, Allergic, Remission Phase EL4 |
| GSM7277547 |
IgE+Monocytes, Allergic, Remission Phase EL5 |
| GSM7277548 |
IgE+Monocytes, Allergic, Remission Phase EL6 |
| GSM7277549 |
IgE+Monocytes, Allergic, Remission Phase EL7 |
| GSM7277550 |
IgE+Monocytes, Healthy, Remission Phase EL8 |
| GSM7277551 |
IgE+Monocytes, Healthy, Remission Phase EL9 |
| GSM7277552 |
IgE+Monocytes, Healthy, Remission Phase EL11 |
| GSM7277553 |
IgE+Monocytes, Healthy, Remission Phase EL13 |
| GSM7277554 |
IgE+Monocytes, Healthy, Remission Phase EL14 |
| GSM7277555 |
IgE+Monocytes, Allergic, Clinical Phase EL15 |
| GSM7277556 |
IgE+Monocytes, Allergic, Clinical Phase EL16 |
| GSM7277557 |
IgE+Monocytes, Allergic, Clinical Phase EL17 |
| GSM7277558 |
IgE+Monocytes, Allergic, Clinical Phase EL18 |
| GSM7277559 |
IgE+Monocytes, Allergic, Clinical Phase EL19 |
| GSM7277560 |
IgE+Monocytes, Allergic, Clinical Phase EL20 |
| GSM7277561 |
IgE+Monocytes, Allergic, Clinical Phase EL21 |
| GSM7277562 |
IgE+Monocytes, Healthy, Clinical Phase EL22 |
| GSM7277563 |
IgE+Monocytes, Healthy, Clinical Phase EL23 |
| GSM7277564 |
IgE+Monocytes, Healthy, Clinical Phase EL25 |
| GSM7277565 |
IgE+Monocytes, Healthy, Clinical Phase EL27 |
| GSM7277566 |
IgE+Monocytes, Healthy, Clinical Phase EL28 |
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| Relations |
| BioProject |
PRJNA965804 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231427_RAW.tar |
2.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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