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| Status |
Public on Dec 20, 2023 |
| Title |
Effect of Parenteral Trace Element Supplementation on RNA-sequencing Profile of Peripheral Blood in Peripartum Dairy Cows |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The transition period is the most critical stage in the lactation cycle of dairy cattle. During this period, cows are subjected to high levels of oxidative stress. One way of managing this stress is through mineral supplementation with antioxidant micronutrients. The aim of this study was to evaluate the gene expression of transition dairy cows supplemented with the antioxidant trace elements copper (Cu), zinc (Zn), manganese (Mn) and selenium (Se). The study was carried out in a commercial Holstein dairy farm located in General Belgrano, province of Buenos Aires, Argentina. Cows (n=200) were randomly assigned to either a supplemented or a control group. Blood samples were obtained seven days after calving and used to determine superoxide dismutase and glutathione peroxidase activity, antioxidant capacity and thiobarbituric acid reactive substances. Additionally, RNA-sequencing analysis was performed. The oxidative stress index differed significantly between groups, despite only two differentially expressed genes which codify for second messengers (adjusted p value < 0.05). This would suggest that trace mineral supplementation of transition dairy cows would not induce changes in gene expression profiles in pathways associated with oxidative stress and immune function, since their expression is already high in response to the high oxidative stress levels and the dietary changes associated with this period. Nevertheless, considering the role of these minerals as cofactors, a higher availability in the supplemented group would increase antioxidant enzyme activity.
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| Overall design |
Transcriptomic profile of peripheral blood in Holstein cows in the transition period that had been supplemented and control animals
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| Contributor(s) |
Calcaterra F, Fernandez ME, Olivera LH, Guzman LA, Giovambattista G, Picco SJ |
| Citation missing |
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| Submission date |
May 02, 2023 |
| Last update date |
Feb 20, 2024 |
| Contact name |
Guillermo Giovambattista |
| E-mail(s) |
guillermogiovambattista@gmail.com
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| Phone |
02214211799
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| Organization name |
CONICET-UNLP
|
| Department |
Facultad de Ciencias Veterinarias
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| Lab |
IGEVET
|
| Street address |
Calle 60 y 118 S/N
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| City |
La Plata |
| State/province |
Buenos Aires |
| ZIP/Postal code |
1900 |
| Country |
Argentina |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA966145 |
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