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| Status |
Public on Jul 27, 2023 |
| Title |
Massively-parallel Microbial mRNA Sequencing (M3-Seq) reveals heterogenous behaviors in bacteria at single-cell resolution |
| Organisms |
Pseudomonas aeruginosa; Escherichia coli; Bacillus subtilis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
M3-Seq is a single-cell RNA-sequencing platform for bacteria that pairs combinatorial cell indexing with post hoc rRNA depletion. We use M3-Seq to profile hundreds of thousands of bacterial cells and reveal rare populations of bacteria that include bet-hedging strategies, prophage induction, and phage-infected cells in E. coli and B. subtilis.
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| Overall design |
Bacterial cells were grown and treated as described in the M3-Seq manuscript. Each named experiment corresponds to a different dataset.
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| Contributor(s) |
Wang B, Lin AE, Yuan J, Koch MD, Adamson BS, Wingreen N, Gitai Z |
| Citation(s) |
37653008 |
| Submission date |
May 08, 2023 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Bruce Wang |
| E-mail(s) |
bw21@princeton.edu
|
| Organization name |
Prinnceton University
|
| Street address |
Lewis Thomas Lab
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08540 |
| Country |
USA |
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| Platforms (2) |
| GPL33390 |
Illumina NovaSeq 6000 (Bacillus subtilis; Escherichia coli) |
| GPL33391 |
Illumina NovaSeq 6000 (Bacillus subtilis; Escherichia coli; Pseudomonas aeruginosa) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA970263 |
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