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| Status |
Public on May 08, 2023 |
| Title |
A versatile nuclei extraction protocol for single nucleus sequencing in non-model species - optimization in various Atlantic salmon tissues |
| Organism |
Salmo salar |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The use of single cell sequencing technologies has exploded in non-model organisms, including fish and invertebrates in an aquaculture setting. One of the most expanding areas is the use sequencing nucleus from frozen tissues allowing being able to sample and sequence at different sites. Here we present a tested nucleus isolation protocol that outperforms the most reliable methods to extract nuclei (chopping in salt tween buffer) and improves its performance in non-model organisms.
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| Overall design |
A versatile nuclei extraction protocol for single nucleus sequencing in fish species – optimization in various Atlantic salmon tissues. dx.doi.org/10.17504/protocols.io.261genwm7g47/v2
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| Contributor(s) |
Ruiz Daniels R, Taylor R, Dobie R, Salisbury S, Clark E, Macqueen D, Robledo D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 08, 2023 |
| Last update date |
May 11, 2023 |
| Contact name |
rose ruiz daniels |
| E-mail(s) |
rose.daniels@roslin.ed.ac.uk
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| Phone |
07469699108
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| Organization name |
The University of Edinburgh
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| Street address |
The University Of Edinburgh
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| City |
Roslin |
| State/province |
NA |
| ZIP/Postal code |
EH259RG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24981 |
Illumina NovaSeq 6000 (Salmo salar) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA970284 |
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