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| Status |
Public on Oct 24, 2023 |
| Title |
Chronic Active Epstein-Barr Virus Disease Originates from Infected Hematopoietic Stem Cells |
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chronic active Epstein-Barr Virus (EBV) disease (CAEBV) is lethal syndrome due to persistent EBV infection. When diagnosed as CAEBV, EBV infection was observed in multiple hematopoietic lineages, but the etiology of CAEBV is still elusive. Bone marrow and peripheral cells derived from five CAEBV patients, one EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH) patient, and two healthy controls were analyzed. Multiple assays were applied to identify and characterize EBV-infected cells, including quantitative PCR (qPCR), PrimeFlow and single-cell RNA-sequencing (scRNA-seq). Based on scRNA-seq data, alterations in gene expression of particular cell types were analyzed between CAEBV patients and controls, and between infected and uninfected cells. One CAEBV patient was treated with allogeneic hematopoietic stem cell transplantation (HSCT), and the samples derived from this patient were analyzed again six month after HSCT. EBV infected the full spectrum of the hematopoietic system including both lymphoid and myeloid lineages, so as hematopoietic stem cells (HSCs) in the CAEBV patients. EBV-infected HSCs exhibited a higher differentiation rate towards downstream lineages, and the EBV infection had an impact on both the innate and adaptive immunity, resulting in inflammatory symptoms. EBV infected cells were thoroughly removed from the hematopoietic system after HSCT. Taken together, multiple lines of evidence presented in this study suggest that CAEBV disease originates from the infected hematopoietic stem cells.
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| Overall design |
We analyzed bone marrow and peripheral cells derived from CAEBV patients and control donors with multiple single-cell RNA-sequencing (scRNA-seq) for both human transcriptomes and EBV-encoded RNA.
NOTE FROM SUBMITTER: We have sensitive patient data here and we want to submit only processed scRNA-seq output files. We are at the moment submitting the raw data to some other databank.
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| Contributor(s) |
Wang J, Su M, Wang X, Wang Z |
| Citation(s) |
37824804 |
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| Submission date |
May 08, 2023 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Xi Wang |
| Organization name |
Nanjing Medical University
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| Street address |
Longmian Avenue 101, Jiangning District
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| City |
Nanjing |
| ZIP/Postal code |
21116 |
| Country |
China |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL33393 |
Illumina NovaSeq 6000 (Human gammaherpesvirus 4) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA970288 |
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