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| Status |
Public on Jan 01, 2012 |
| Title |
Expression data in single and double knockouts of AtNHX5 and AtNHX6 in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
We generated single and double knockouts of AtNHX5 and AtNHX6 in order to invesigate possible functions in Arabidopsis. nhx5/nhx6 exhibited severe growth retardation related to cell size and proliferation, as well as endosomal trafficking perutrbations. The results implicate endosomal NHX antiporters in novel cellular functions. In order to investigate further the possible functions of AtNHX5 and AtNHX6, we compared the transcrptional profiles of single and double AtNHX5 and AtNHX6 knockouts. We looked for changes in gene expression might help us to elucidate the molecular events associated with the apparent requirement of AtNHX5 and AtNHX6 for normal growth and development.
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| Overall design |
Rosette leaves from 4 weeks old plants grown on soil under normal conditons and 8h L / 16 D were collected for RNA extraction and hybridization on Affymetrix microarray chips.
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| Contributor(s) |
Blumwald E, Bassil E |
| Citation(s) |
21278129 |
| Submission date |
Jul 27, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
elias bassil |
| E-mail(s) |
esbassil@ucdavis.edu
|
| Organization name |
university of california
|
| Department |
plant sciences
|
| Lab |
blumwald
|
| Street address |
1 shields ave
|
| City |
davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (15)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA131633 |
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