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| Status |
Public on May 01, 2024 |
| Title |
The phosphorylation of FOXA1-S331 is indispensable for FOXA1-AR dependent cistrome [Cut&Tag] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
To define the function of FOXA1-S331 phosphorylation, we substituted S331 in both FOXA1 alleles for alanine in C4-2 cells via CRISPR/Cas9-based gene editing, thereby generating phosphorylation incompetent FOXA1S331A cells. We directly assessed the consequences of phospho-incompetent FOXA1S331A mutation on the transcription profile, chromatin deposition of FOXA1, AR, H3K27ac and chromatin accessibility.
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| Overall design |
Chromatin immunoprecipitation DNA-sequencing (CUT&Tag-seq) for FOXA1, AR well as the histone modification H3K27ac in WT and FOXA1S331A C4-2 cells.
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| Contributor(s) |
Guo J, Qin J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 10, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Ni Li |
| E-mail(s) |
lini@sibs.ac.cn
|
| Organization name |
Institute of Health Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences
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| Lab |
Laboratory of Tumor Progression and Metastasis
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| Street address |
320 Yueyang Road, Shanghai, 200025 P.R. China
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE232125 |
The phosphorylation of FOXA1-S331 is indispensable for FOXA1-AR dependent cistrome. |
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| Relations |
| BioProject |
PRJNA971092 |
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