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Series GSE232123 Query DataSets for GSE232123
Status Public on May 01, 2024
Title The phosphorylation of FOXA1-S331 is indispensable for FOXA1-AR dependent cistrome [Cut&Tag]
Organism Homo sapiens
Experiment type Other
Summary To define the function of FOXA1-S331 phosphorylation, we substituted S331 in both FOXA1 alleles for alanine in C4-2 cells via CRISPR/Cas9-based gene editing, thereby generating phosphorylation incompetent FOXA1S331A cells. We directly assessed the consequences of phospho-incompetent FOXA1S331A mutation on the transcription profile, chromatin deposition of FOXA1, AR, H3K27ac and chromatin accessibility.
Overall design Chromatin immunoprecipitation DNA-sequencing (CUT&Tag-seq) for FOXA1, AR well as the histone modification H3K27ac in WT and FOXA1S331A C4-2 cells.
Contributor(s) Guo J, Qin J
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Submission date May 10, 2023
Last update date May 01, 2024
Contact name Ni Li
Organization name Institute of Health Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences
Lab Laboratory of Tumor Progression and Metastasis
Street address 320 Yueyang Road, Shanghai, 200025 P.R. China
City Shanghai
State/province Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM7315569 WT_FOXA1
GSM7315570 MUT_FOXA1
GSM7315571 WT_AR
This SubSeries is part of SuperSeries:
GSE232125 The phosphorylation of FOXA1-S331 is indispensable for FOXA1-AR dependent cistrome.
BioProject PRJNA971092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE232123_RAW.tar 878.0 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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