|
Status |
Public on May 15, 2023 |
Title |
Rat cytomegalovirus efficiently replicates within dendritic cells and affects both surface marker and gene expression |
Organisms |
Rattus norvegicus; Murid betaherpesvirus 8 |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Dendritic cells (DC) play a crucial role in generating and maintaining antiviral immunity. While DC are implicated in the antiviral defense by inducing T cell responses, they can also become infected by Cytomegalovirus (CMV). CMV is not only highly species-specific but also specialized in evading immune protection, and this specialization is in part due to characteristic genes encoded by a given virus. Here, we investigated whether RCMV can infect XCR1+ DC and if infection of DC alters the expression of cell surface markers and migration behavior. We demonstrate that wild-type RCMV and a _vxcl1 mutant virus infect splenic rat DC ex vivo and identify viral assembly compartments. Replication-competent RCMV reduced XCR1 and MHCII surface expression. Further, gene expression of infected DC was analyzed by single cell RNA-sequencing. RCMV infection reverted a state of DC activation that was induced by DC cultivation. On the functional level, we observed impaired chemotactic activity of infected XCR1+ DC compared to mock treated cells. We therefore speculate that as a result of RCMV infection, DC exhibit diminished XCR1 expression and are thereby inhibited from the lymphocyte crosstalk.
|
|
|
Overall design |
Dendritic cells were isolated from rat spleens, and RNA extracted immediately (input samples) or after 24 hours of treatment as indicated.
|
|
|
Contributor(s) |
Madela-Mönchinger JC, Wolf SA, Wyler E, Bauer A, Möller L, Juranić Lisnić V, Landthaler M, Malyshkina A, Voigt S |
Citation(s) |
38077365 |
|
Submission date |
May 10, 2023 |
Last update date |
Feb 22, 2024 |
Contact name |
Emanuel Wyler |
E-mail(s) |
emanuel.wyler@mdc-berlin.de
|
Phone |
+49 30 9406 3009
|
Organization name |
Max Delbrück Center for Molecular Medicine
|
Department |
Berlin Institute for Medical Systems Biology
|
Lab |
RNA Biology and Posttranscriptional Regulation
|
Street address |
Robert Roessle Str 10
|
City |
Berlin |
ZIP/Postal code |
13125 |
Country |
Germany |
|
|
Platforms (2) |
GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
GPL34220 |
Illumina HiSeq 4000 (Murid betaherpesvirus 8; Rattus norvegicus) |
|
Samples (15)
|
|
Relations |
BioProject |
PRJNA971185 |