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| Status |
Public on May 15, 2023 |
| Title |
Rat cytomegalovirus efficiently replicates within dendritic cells and affects both surface marker and gene expression |
| Organisms |
Rattus norvegicus; Murid betaherpesvirus 8 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dendritic cells (DC) play a crucial role in generating and maintaining antiviral immunity. While DC are implicated in the antiviral defense by inducing T cell responses, they can also become infected by Cytomegalovirus (CMV). CMV is not only highly species-specific but also specialized in evading immune protection, and this specialization is in part due to characteristic genes encoded by a given virus. Here, we investigated whether RCMV can infect XCR1+ DC and if infection of DC alters the expression of cell surface markers and migration behavior. We demonstrate that wild-type RCMV and a _vxcl1 mutant virus infect splenic rat DC ex vivo and identify viral assembly compartments. Replication-competent RCMV reduced XCR1 and MHCII surface expression. Further, gene expression of infected DC was analyzed by single cell RNA-sequencing. RCMV infection reverted a state of DC activation that was induced by DC cultivation. On the functional level, we observed impaired chemotactic activity of infected XCR1+ DC compared to mock treated cells. We therefore speculate that as a result of RCMV infection, DC exhibit diminished XCR1 expression and are thereby inhibited from the lymphocyte crosstalk.
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| Overall design |
Dendritic cells were isolated from rat spleens, and RNA extracted immediately (input samples) or after 24 hours of treatment as indicated.
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| Contributor(s) |
Madela-Mönchinger JC, Wolf SA, Wyler E, Bauer A, Möller L, Juranić Lisnić V, Landthaler M, Malyshkina A, Voigt S |
| Citation(s) |
38077365 |
| |
| Submission date |
May 10, 2023 |
| Last update date |
Feb 22, 2024 |
| Contact name |
Emanuel Wyler |
| E-mail(s) |
emanuel.wyler@mdc-berlin.de
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| Phone |
+49 30 9406 3009
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| Organization name |
Max Delbrück Center for Molecular Medicine
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| Department |
Berlin Institute for Medical Systems Biology
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| Lab |
RNA Biology and Posttranscriptional Regulation
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| Street address |
Robert Roessle Str 10
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| City |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
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| Platforms (2) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
| GPL34220 |
Illumina HiSeq 4000 (Murid betaherpesvirus 8; Rattus norvegicus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA971185 |
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