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| Status |
Public on Jun 01, 2011 |
| Title |
CpG islands Methylation Profiling by DMH in Epithelial Ovarian Cancer: Screening Set |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
We have systematically profiled DNA methylation at promoter CpG islands (CGIs) in ovarian cancer. Epithelial ovarian tumours, excluding mucinous and clear cell cancers, prospectively collected through a cohort study, were analyzed by differential methylation hybridization (DMH) (Nouzova M et al, 2004) in duplicates. The loci targeted by the custom-designed microarray are the promoter CpG islands (Gardiner-Garden and Frommer, 1987) of the genes involved in the Wnt, p53, AKT/mTOR, BRCA1/2 and Redox pathways, DNA repair (HR, NHEJ and MMR), FA family and IgLON family.
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| Overall design |
111 ovarian tumor samples were assayed by DMH in duplicates. McrBC digested (Cy5) and undigested (Cy3) samples were competitively hybridized on the Agilent custom-designed microarrays 8x15k.
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| Contributor(s) |
Dai W, Teodoridis JM, Zeller C, Graham JS, Hersey JM, Flanagan JM, Stronach EA, Millan DW, Siddiqui N, Paul J, Brown R |
| Citation(s) |
21459799 |
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| Submission date |
Jul 28, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Wei Dai |
| E-mail(s) |
w.dai@imperial.ac.uk
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| Organization name |
Imperial College London
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| Department |
Sugery and Cancer
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| Lab |
Epigenetics
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| Street address |
Du Cane Road
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| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10734 |
Agilent custom-designed CpG island 60mer microarray 8x15k |
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| Samples (222)
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| Relations |
| BioProject |
PRJNA131437 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23240_RAW.tar |
996.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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