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Series GSE23250 Query DataSets for GSE23250
Status Public on Aug 05, 2010
Title L3-L4_PVD_OLL_neuron_tiling_array
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Expression profiling by genome tiling array
Summary modENCODE_submission_460
This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see
Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
For data usage terms and conditions, please refer to and

Keywords: Transcript tiling array analysis
Overall design EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: NC1021 (engineered, target gene ser-2 tagged by 3xFlag::PAB-1); Tissue: PVD OLLs (L3-L4); Developmental Stage: L3-L4 larva 20dC 22h 23dC 24hr post-L1; Genotype: unc-119 (ed1); wdEx460 [unc-119 (+); ser-2prom3B::3XFLAG::PAB-1]; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAGĀ® M2 Affinity Gel (target is FLAG); Strain NC1021 (engineered, target gene ser-2 tagged by 3xFlag::PAB-1); temperature 23; Developmental Stage L3-L4 larva 20dC 22h 23dC 24hr post-L1; Tissue PVD OLLs (L3-L4)
Web link
Contributor(s) Waterston B, Miller D, Gerstein M, Wilson M, Argiwal A, Reinke V
Citation(s) 21177967
BioProject PRJNA168961
Submission date Jul 28, 2010
Last update date May 02, 2013
Contact name DCC modENCODE
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
Platforms (1)
GPL5634 [Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array
Samples (3)
GSM571431 L3-L4 PVD & OLL neurons tiling array extraction1_array1
GSM571432 L3-L4 PVD & OLL neurons tiling array extraction2_array1
GSM571433 L3-L4 PVD & OLL neurons tiling array extraction3_array1

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23250_L3-L4-PVD_OLL_smooth_log2_winsize110.bedgraph.gz 26.9 Mb (ftp)(http) BEDGRAPH
GSE23250_L3-L4-PVD_OLL_tars_maxgap30_minrun100_thresh7.00.gff3.gz 1.1 Mb (ftp)(http) GFF3
GSE23250_RAW.tar 117.2 Mb (http)(custom) TAR (of CEL)
Processed data are available on Series record

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