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| Status |
Public on Dec 22, 2023 |
| Title |
Impact of the vitamin B12 on the transcriptional regulation of the Mycobacterium tuberculosis Complex |
| Organisms |
Mycobacterium tuberculosis variant bovis; Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The transcriptome in response to vitamin B12 was interrogated in three M. tuberculosis Complex species: M. tuberculosis H37Rv, M. tuberculosis GC1237, and M. bovis AF2122/97. Examination of the core B12 transcriptome resulted in a robust differential regulation of 7 genes in the three species analysed
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| Overall design |
Each Mycobacterium tuberculosis heterotypic synonym (M. tuberculosis H37Rv, M. tuberculosis GC1237, and M. bovis AF2122/97) was grown in standard laboratory media supplemented with or without vitamin B12. Two replicates of each strain were processed in each condition (2 with B12 and 2 without B12 for each strain). RNA was extracted and subjected to RNA-seq. Expression profiles for each DNA strand (plus and minus) were obtained for the three species analysed.
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| Web link |
https://www.nature.com/articles/s41467-024-46449-8
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| Contributor(s) |
Gonzalo-Asensio J, Campos-Pardos E |
| Citation(s) |
38461302 |
| |
| Submission date |
May 17, 2023 |
| Last update date |
Mar 22, 2024 |
| Contact name |
Jesus Gonzalo-Asensio |
| E-mail(s) |
jagonzal@unizar.es
|
| Organization name |
University of Zaragoza
|
| Department |
Microbiology
|
| Lab |
Grupo de Genética de Micobacterias
|
| Street address |
C/ Domingo Miral sn
|
| City |
Zaragoza |
| State/province |
Zaragoza |
| ZIP/Postal code |
50009 |
| Country |
Spain |
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| Platforms (2) |
| GPL17879 |
Illumina HiSeq 2500 (Mycobacterium tuberculosis) |
| GPL26660 |
Illumina HiSeq 2500 (Mycobacterium tuberculosis variant bovis) |
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| Samples (12)
|
| GSM7371846 |
M. tuberculosis H37Rv without B12 replicate 1 |
| GSM7371847 |
M. tuberculosis H37Rv without B12 replicate 2 |
| GSM7371848 |
M. tuberculosis H37Rv with B12 replicate 1 |
| GSM7371849 |
M. tuberculosis H37Rv with B12 replicate 2 |
| GSM7371850 |
M. tuberculosis GC1237 without B12 replicate 1 |
| GSM7371851 |
M. tuberculosis GC1237 without B12 replicate 2 |
| GSM7371852 |
M. tuberculosis GC1237 with B12 replicate 1 |
| GSM7371853 |
M. tuberculosis GC1237 with B12 replicate 2 |
| GSM7371854 |
M. bovis AF2122/97 without B12 replicate 1 |
| GSM7371855 |
M. bovis AF2122/97 without B12 replicate 2 |
| GSM7371856 |
M. bovis AF2122/97 with B12 replicate 1 |
| GSM7371857 |
M. bovis AF2122/97 with B12 replicate 2 |
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| Relations |
| BioProject |
PRJNA973593 |
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