|
| Status |
Public on Nov 29, 2023 |
| Title |
Clostridioides difficile toxins deregulate tight junction proteins. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The Clostridioides difficile toxins TcdA and TcdB are responsible for diarrhea and colitis. The aim of this project was to explore the effects of the toxins on epithelial barrier function and the molecular mechanisms for diarrhea and inflammation. RNA-seq of toxin-treated intestinal cell monolayers was performed to describe the C. difficile-mediated effects. mRNA profiles from intestinale epithelial cells were generated by deep sequencing using Illumina NovaSeq 6000. This data provide the basis for subsequent upstream regulator analysis.
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| |
|
| Overall design |
mRNA profiles from 3 TcdA-treated, 3 TcdB-treated and 3 control cell monolayers were generated by paired-end sequencing using Illumina NovaSeq 6000.
|
| |
|
| Contributor(s) |
Schneemann M, Heils L, Moos V, Weiner J, Beule D, Gerhard R, Schulzke J, Bücker R |
| Citation(s) |
37999506 |
| |
| Submission date |
May 17, 2023 |
| Last update date |
Nov 29, 2023 |
| Contact name |
January 3rd Weiner |
| E-mail(s) |
january.weiner@gmail.com
|
| Phone |
030450543049
|
| Organization name |
Berlin Institute of Health, Charité Medical University of Berlin
|
| Department |
CUBI
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
|
| GSM7372106 |
P2017_RNA_01HT-29/B6-GR/MRcontrol [9398_S117] |
| GSM7372107 |
P2017_RNA_02HT-29/B6-GR/MRcontrol [9399_S118] |
| GSM7372108 |
P2017_RNA_03HT-29/B6-GR/MRcontrol [9400_S119] |
| GSM7372109 |
P2017_RNA_04HT-29/B6-GR/MRTcdA-treated [9401_S120] |
| GSM7372110 |
P2017_RNA_05HT-29/B6-GR/MRTcdA-treated [9402_S121] |
| GSM7372111 |
P2017_RNA_06HT-29/B6-GR/MRTcdA-treated [9403_S122] |
| GSM7372112 |
P2017_RNA_07HT-29/B6-GR/MRTcdB-treated [9404_S123] |
| GSM7372113 |
P2017_RNA_08HT-29/B6-GR/MRTcdB-treated [9405_S124] |
| GSM7372114 |
P2017_RNA_09HT-29/B6-GR/MRTcdB-treated [9406_S125] |
|
| Relations |
| BioProject |
PRJNA973611 |
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